Behind the scenes of a small RNA gene-silencing pathway

Human Gene Therapy
Gregory Ku, Michael McManus

Abstract

RNA interference (RNAi) is a sequence-specific gene-silencing phenomenon mediated by double-stranded RNA. RNAi is made possible through the activity of an evolutionarily conserved RNA-protein machinery that is utilized by microRNAs (miRNAs), endogenously produced 21- to 23-nucleotide noncoding RNAs. As RNA-based therapeutics come of age, understanding the miRNA pathway and the mechanism of posttranscriptional gene silencing is paramount. Studies have pointed to a critical role for miRNAs in human development and disease and revealed an unanticipated complexity in the mechanisms of regulation of gene expression. This review discusses advances in the biogenesis of miRNAs, their modes of action, and implications for RNA-based treatments.

References

Jan 5, 2000·Genes & Development·T TuschlP A Sharp
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Apr 18, 2002·Genes & Development·Patrick J PaddisonDouglas S Conklin
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Guangchao SuiYang Shi
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jenn-Yah YuDavid L Turner
May 1, 2002·Nature Biotechnology·Cynthia P PaulDavid R Engelke
Jun 29, 2002·RNA·Michael T McManusPhillip A Sharp
Aug 29, 2002·The EMBO Journal·Yoontae LeeV Narry Kim
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael T McManusPhillip A Sharp
Jan 30, 2003·RNA·Mariana Lagos-QuintanaThomas Tuschl
Feb 26, 2003·Genes & Development·John G DoenchPhillip A Sharp
May 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jen-Tsan ChiPatrick O Brown
May 20, 2003·Nature Biotechnology·Aimee L JacksonPeter S Linsley
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Oct 7, 2003·Nature Genetics·Emily BernsteinGregory J Hannon
Oct 22, 2003·Cell·Dianne S SchwarzPhillip D Zamore
Oct 22, 2003·Cell·Anastasia KhvorovaSumedha D Jayasena
Nov 25, 2003·Science·Elsebet LundUlrike Kutay
Dec 19, 2003·Genes & Development·Rui YiBryan R Cullen
Mar 12, 2004·Genes & Development·John G Doench, Phillip A Sharp
Apr 24, 2004·Science·Soraya YektaDavid P Bartel
Jul 20, 2004·The Plant Cell·Ramanjulu Sunkar, Jian-Kang Zhu
Sep 15, 2004·Genome Research·Antony RodriguezAllan Bradley
Nov 6, 2004·Science·Binzhang Shen, Howard M Goodman
Nov 9, 2004·Nature·Richard I GregoryRamin Shiekhattar
Nov 13, 2004·Nature·Matthew N PoyMarkus Stoffel
Nov 20, 2004·Science·Yukihide TomariPhillip D Zamore
Dec 3, 2004·Genes & Development·Jinju HanV Narry Kim

❮ Previous
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Citations

Oct 27, 2009·Journal of the American Society for Mass Spectrometry·Teng-yi HuangScott A McLuckey
Oct 28, 2008·Seminars in Reproductive Medicine·Tannaz ToloubeydokhtiNasser Chegini
Oct 28, 2008·Seminars in Reproductive Medicine·Xiaoping Luo, Nasser Chegini
Nov 4, 2011·Antioxidants & Redox Signaling·Mukesh Verma
Mar 5, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·O KhorramW J Pearce
Aug 28, 2012·Comparative and Functional Genomics·Tenzin W Lhakhang, M Ahmad Chaudhry
Oct 28, 2011·PloS One·Robert Jan LebbinkMichael T McManus
Mar 7, 2014·Journal of Zhejiang University. Science. B·Zi-ming YuanQi Zheng
Dec 6, 2011·Pulmonary Circulation·Sachindra R JoshiWilliam T Gerthoffer
Mar 28, 2020·Cardiovascular Research·Luca BragaMauro Giacca

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