Benchtop and animal validation of a portable fluorescence microscopic imaging system for potential use in cholecystectomy

Journal of Biomedical Optics
Jian YeRonald X Xu

Abstract

We propose a portable fluorescence microscopic imaging system (PFMS) for intraoperative display of biliary structure and prevention of iatrogenic injuries during cholecystectomy. The system consists of a light source module, a camera module, and a Raspberry Pi computer with an LCD. Indocyanine green (ICG) is used as a fluorescent contrast agent for experimental validation of the system. Fluorescence intensities of the ICG aqueous solution at different concentration levels are acquired by our PFMS and compared with those of a commercial Xenogen IVIS system. We study the fluorescence detection depth by superposing different thicknesses of chicken breast on an ICG-loaded agar phantom. We verify the technical feasibility for identifying potential iatrogenic injury in cholecystectomy using a rat model in vivo. The proposed PFMS system is portable, inexpensive, and suitable for deployment in resource-limited settings.

References

Jun 11, 2004·Journal of Biomedical Optics·Baohong YuanQuing Zhu
Mar 27, 2007·Journal of the American College of Surgeons·Nader N Massarweh, David R Flum
Aug 9, 2013·Nature Reviews. Cancer·Quyen T Nguyen, Roger Y Tsien

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