PMID: 2107318Feb 20, 1990Paper

Bend induced by the phage phi 29 transcriptional activator in the viral late promoter is required for activation

Journal of Molecular Biology
F RojoM Salas

Abstract

Transcription initiation from the Bacillus subtilis phage phi 29 late A3 promoter requires the viral protein p4, a transcriptional activator. Protein p4 binds to a region of the A3 promoter, located between nucleotides -50 and -100 relative to the transcription start site, that presents a sequence-directed curvature. This curvature is enhanced when protein p4 binds to the promoter. A number of deletion mutants at the carboxyl end of protein p4 have been constructed and their behavior as transcriptional activators of the late A3 promoter has been investigated. The binding of these deletion mutants to the late A3 promoter has been analyzed by gel retardation, DNase I footprinting, methylation interference and circular permutation assays. The results suggest that the last 12 amino acid residues of protein p4, six of which are positively charged, although not involved in the specific recognition of the promoter are responsible for part of the bend induced by protein p4 in its binding site. Evidence is presented which suggests that full induction of this curvature is needed for the transcription activation process. A model is proposed for protein p4 interaction with the A3 promoter, in which the bend is induced in two steps: first, ...Continue Reading

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Citations

Jan 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P C van der Vliet, C P Verrijzer
Sep 1, 1991·Research in Microbiology·F RojoM Salas
Aug 6, 1998·Biophysical Chemistry·J K Strauss-SoukupL J Maher
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B NuezM Salas
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·G del SolarM Espinosa
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MencíaM Salas
Feb 20, 2004·Nucleic Acids Research·Wilfried J J Meijer, Margarita Salas
Jan 12, 2005·Nucleic Acids Research·Laura Pérez-LagoAna Camacho
Sep 18, 2007·Journal of Bacteriology·Martín AlcorloJosé M Hermoso
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·W J MeijerM Salas
Jan 1, 1997·Annual Review of Microbiology·J Pérez-Martín, V de Lorenzo
Apr 24, 2010·FEMS Microbiology Reviews·Ana Camacho, Margarita Salas
Sep 1, 1991·Molecular & General Genetics : MGG·J C Alonso, A C Stiege
Feb 12, 1998·The Journal of Biological Chemistry·J K Strauss-Soukup, L J Maher
Jun 1, 1994·Microbiological Reviews·J Pérez-MartínV de Lorenzo
Nov 22, 1991·Science·T K Kerppola, T Curran
May 7, 1993·Science·J Pérez-Martín, M Espinosa
May 25, 1990·Science·M SerranoJ M Hermoso

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