Bending of protonema cells in a plastid glycolate/glycerate transporter knockout line of Physcomitrella patens

PloS One
Jin NakaharaH Takano

Abstract

Arabidopsis LrgB (synonym PLGG1) is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). We isolated two homologous genes (PpLrgB1 and B2) from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB-GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2) and double (∆B1/∆B2)-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, protonema cells of wil...Continue Reading

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Citations

Mar 20, 2016·Journal of Experimental Botany·Younès DelleroMichael Hodges
Dec 13, 2016·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yoshiko TakahashiHiroyoshi Takano
Aug 7, 2018·Plant Science : an International Journal of Experimental Plant Biology·Shiwen ZhangJunhong Zhang

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Methods Mentioned

BETA
light stress
phosphotransferase
gene knockout
PCR

Software Mentioned

MEGA5
TargetP
SPSS
tBlastN

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