Beneficial effect of the oligomerized polyphenol oligonol on high glucose-induced changes in eNOS phosphorylation and dephosphorylation in endothelial cells.

British Journal of Pharmacology
Xiao-Hong ZhangYuichi Hattori

Abstract

Hyperglycaemia is known to reduce nitric oxide (NO) bioavailability by modulating endothelial NO synthase (eNOS) activity, and polyphenols are believed to have cardiovascular benefit. One possible mechanism could be through interaction with eNOS. The effects of the oligomerized polyphenol oligonol on eNOS phosphorylation status and activity were examined in porcine aortic endothelial cells cultured in high glucose concentrations. Exposure to high glucose concentrations strongly inhibited eNOS phosphorylation at Ser-1177 and dephosphorylation at Thr-495 in bradykinin (BK)-stimulated cells. These inhibitory effects of high glucose were significantly prevented by treatment with oligonol. Akt and p38 mitogen-activated protein kinase (MAPK) were activated in BK-stimulated cells. High glucose inhibited Akt activation but enhanced p38 MAPK activation, both of which were reversed by oligonol treatment. The phosphatidylinositol 3-kinase inhibitor wortmannin blocked the reversal by oligonol of phosphorylation at Ser-1177, but not dephosphorylation at Thr-495, in BK-stimulated cells exposed to high glucose. The effect of oligonol on BK dephosphorylation under high glucose was mimicked by protein kinase C (PKC) epsilon-neutralizing peptide...Continue Reading

References

Mar 1, 1997·British Journal of Pharmacology·E AndriambelosonR Andriantsitohaina
Oct 6, 1998·Annual Review of Biochemistry·D A FrumanL C Cantley
Sep 2, 1999·Current Biology : CB·B J MichellR B Pearson
Jul 14, 2000·American Journal of Physiology. Endocrinology and Metabolism·Y DingD D Roh
Sep 23, 2000·American Journal of Physiology. Endocrinology and Metabolism·W LiuW L Lowe
Nov 14, 2000·Circulation Research·H Cai, D G Harrison
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H TomiokaM Kanno
Feb 24, 2001·American Journal of Physiology. Renal Physiology·R Govers, T J Rabelink
Apr 5, 2001·American Journal of Physiology. Cell Physiology·F KimM A Corson
Dec 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·Bruno SchnyderSilvia Schnyder-Candrian
Dec 11, 2002·The Journal of Pathology·Ester W J A AlbrechtHarry van Goor
Jan 4, 2003·The Journal of Biological Chemistry·Chieko MineoPhilip W Shaul
May 7, 2003·Biochemical and Biophysical Research Communications·Hiroshi TakaishiMitsuhiro Yokoyama
Jun 5, 2003·American Journal of Physiology. Endocrinology and Metabolism·S McGinnC A Pollock
May 14, 2004·American Journal of Physiology. Renal Physiology·Shaoyou Chu, H Glenn Bohlen
Aug 31, 2004·The Journal of Biological Chemistry·Elad AnterJohn F Keaney
Jan 22, 2005·Annals of the New York Academy of Sciences·Mei-Hua LiYoung-Joon Surh
Sep 9, 2006·Bioscience, Biotechnology, and Biochemistry·Hajime FujiiGen-ichiro Nonaka
Nov 4, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hajime FujiiOkezie I Aruoma
Jan 18, 2007·American Journal of Physiology. Endocrinology and Metabolism·Maria A PotenzaMonica Montagnani
Jul 25, 2007·Journal of Internal Medicine·M BartnikL Rydén

❮ Previous
Next ❯

Citations

Aug 26, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jaemoo ChunYeong Shik Kim
Jun 14, 2012·Circulation Research·Francesco PaneniFrancesco Cosentino
Apr 11, 2015·Nutrition Research and Practice·Jae Hyun AhnEun Ju Cho
Jul 18, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Sang Ki LeeByeong Hwa Jeon
Jun 15, 2012·Toxicology Mechanisms and Methods·Mahesh ThirunavukkarasuManashi Bagchi
Jul 14, 2020·World Journal of Gastroenterology : WJG·Aida KamalianAmir Hossein Abdolghaffari
Sep 24, 2021·Critical Reviews in Food Science and Nutrition·Mamali DasSeyed Mohammad Nabavi

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.