Beneficial effects of evodiamine on P2X(4)-mediated inflammatory injury of human umbilical vein endothelial cells due to high glucose

International Immunopharmacology
Qiulan LvShangdong Liang

Abstract

Evodiamine has been reported to exhibit anti-inflammatory and anti-nociceptive effects, but the underlying mechanisms remain to be defined. P2X4 receptor (P2X4R) is a subtype of ATP receptors and plays important roles in pain, inflammatory and immune responses. We aimed to investigate whether evodiamine has beneficial effects on endothelial inflammatory injury mediated by chronic high glucose condition. We found that culturing human umbilical vein endothelial cells (HUVECs) with high glucose significantly increased the expression of P2X4 receptor in HUVECs, cytosolic Ca(2+) concentrations and intracellular reactive oxygen species (ROS) while decreasing nitric oxide (NO); these effects could be reversed by evodiamine. High glucose also significantly increased the expression of the pro-inflammatory activators (NF-κB) and TNFR-ɑ, which was accompanied by the elevation of P2X4R levels. Evodiamine was able to down-regulate the elevated NF-κB, TNFR-ɑ, P2X4R and ROS, and up-regulate the decreased NO. Thus the evodiamine may exert the anti-inflammation activity on high-glucose challenge HUVEC via suppressing the P2X4R signaling pathway, exhibiting beneficial ability to protect HUVECs from glucotoxicity.

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Citations

Feb 20, 2018·Archives of Physiology and Biochemistry·Walid Hamdy El-Tantawy, Abeer Temraz
Jul 22, 2017·Molecular Medicine Reports·Jing-Yun ZhangQiu-Mei Zhang
Mar 28, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Yongsong XuZhimin Wang
Apr 2, 2020·Frontiers in Pharmacology·Mahmoud G KhalafallaGary A Weisman
Mar 18, 2017·Molecular and Cellular Biochemistry·Qing-Qing WuQi-Zhu Tang
Jan 9, 2019·Frontiers in Pharmacology·Yiming ChenLei Zhang
Feb 23, 2018·Oncology Letters·Ting XuJun Ouyang
Mar 13, 2021·Pharmacological Reports : PR·Kamal S AbdelrahmanMohamed Abdel-Aziz
Jun 3, 2021·International Journal of Molecular Sciences·Ching-Hsuan Chou, Chia-Ron Yang
Sep 6, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wen-Jun ZhangZheng-Ming Zhu

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