Beneficial effects of intramyocardial mesenchymal stem cells and VEGF165 plasmid injection in rats with furazolidone induced dilated cardiomyopathy

Journal of Cellular and Molecular Medicine
Qin YuXiaofei Li

Abstract

To explore the impact of myocardial injection of mesenchymal stem cells (MSCs) and specific recombinant human VEGF165 (hVEGF165 ) plasmid on collagen remodelling in rats with furazolidone induced dilated cardiomyopathy (DCM). DCM was induced by furazolidone (0.3 mg/bodyweight (g)/day per gavage for 8 weeks). Rats were then divided into four groups: (i) PBS group (n = 18): rats received equal volume myocardial PBS injection; (ii) MSCs group (n = 17): 100 μl culture medium containing 10(5) MSCs were injected into four sites of left ventricular free wall (25 μl per site); (iii) GENE group (n = 18): pCMVen-MLC2v-EGFP-VEGF165 plasmid [5 × 10(9) pfu (0.2 ml)] were injected into four sites of left ventricular free wall (0.05 ml per site)] and (iv) MSCs+GENE group (n = 17): rats received both myocardial MSCs and pCMVen-MLC2v-EGFP-VEGF165 plasmid injections. After 4 weeks, cardiac function was evaluated by echocardiography. Myocardial mRNA expressions of type I, type III collagen and transforming growth factor (TGF)-β1 were detected by RT-PCR. The protein expression of hVEGF165 was determined by Western blot. Myocardial protein expression of hVEGF165 was demonstrated in GENE and MSCs+GENE groups. Cardiac function was improved in MSCs, G...Continue Reading

References

Dec 8, 1994·The New England Journal of Medicine·G W Dec, V Fuster
Jan 8, 1998·BMJ : British Medical Journal·C Oakley
Jun 1, 2002·Journal of the American Society of Nephrology : JASN·Jin H LiHui Y Lan
Aug 21, 2004·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ryang Hwa LeeJin Sup Jung
Sep 7, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Lei YeEugene K W Sim
Apr 20, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kyoji UrayamaCanan G Nebigil
Jul 4, 2007·Clinical Medicine & Research·Valério Garrone BaraunaEdilamar Menezes de Oliveira
Jan 3, 2008·Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·A E LootR H Henning
Mar 18, 2008·Journal of Molecular and Cellular Cardiology·Yukiko ImanishiYoshiki Sawa
Jan 26, 2010·Transplantation·Vera Regitz-ZagrosekRoland Hetzer
Jul 21, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fabio R FormigaMaria J Blanco-Prieto
May 21, 2011·British Medical Bulletin·Abdul M MozidAnthony Mathur
Jul 13, 2011·Molecular and Cellular Biology·Azariyas A Challa, Branko Stefanovic
Oct 27, 2011·Journal of Ethnopharmacology·Nian-Bao ZhangBo-Ping Ding
Apr 12, 2013·Stem Cells International·Devang M PatelAnand S Srivastava
Jun 12, 2013·Cell Stem Cell·Jessica C Garbern, Richard T Lee
Nov 8, 2013·Journal of Translational Medicine·Marianna BellafioreAntonio Paoli

❮ Previous
Next ❯

Citations

Aug 12, 2019·Biochemical and Biophysical Research Communications·Haihong LinHui Gong
Jul 25, 2021·International Journal of Molecular Sciences·Fernanda GubertGisele Zapata-Sudo

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
flow cytometry
light microscopy
PCR
fluorescence-activated cell sorting
electrophoresis

Software Mentioned

Image
SPSS ( Statistical Product and Service Solutions )
Pro Plus

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.