PMID: 20103988Jan 28, 2010Paper

Beneficial effects of reversine on in vitro development of miniature pig somatic cell nuclear transfer embryos

The Journal of Reproduction and Development
Kazuchika MiyoshiMasahiro Sato

Abstract

Reversine, a 2-(4-morpholinoanilino)-6-cyclohexylaminopurine analog, can induce dedifferentiation of myogenic lineage-committed cells into multipotent mesenchymal progenitor cells, from which osteoblasts and adipocytes redifferentiate under lineage-specific inducing conditions. Although the molecular mechanism of how reversine causes dedifferentiation of a differentiated cell has not been fully elucidated, we speculated that it would be involved in reprogramming. In the present study, we examined whether reversine can enhance the development of somatic cell nuclear transfer (SCNT) embryos by improving the reprogramming state of the somatic cell nuclei. As donor cells, we used miniature pig fetal fibroblasts transfected with a plasmid construct containing a mouse Oct-3/4 promoter and enhanced green fluorescent protein (EGFP) cDNA. When the nuclei of these transfected cells are reprogrammed to an undifferentiated state in the SCNT embryos, EGFP expression is expected to commence under the control of the Oct-3/4 promoter. After SCNT, the resulting embryos were treated with 5 muM reversine for different durations (0, 6, 12, 18 and 24 h) or at different concentrations (0, 1, 5 and 10 muM) of reversine for 12 h and then cultured in v...Continue Reading

References

May 15, 1997·Genes & Development·A K Ryan, M G Rosenfeld
Apr 20, 1999·Biology of Reproduction·M J van EijkF Gandolfi
Nov 25, 2000·Biology of Reproduction·N KirchhofH Niemann
Mar 10, 2001·Molecular Reproduction and Development·T Wakayama, R Yanagimachi
Jun 13, 2003·Biology of Reproduction·Kimiko InoueAtsuo Ogura
Jan 15, 2004·Journal of the American Chemical Society·Shuibing ChenSheng Ding
Aug 12, 2005·Molecular Reproduction and Development·Keisuke SatoKazuchika Miyoshi
Oct 13, 2005·Cloning·P A De SousaM E Westhusin
Dec 17, 2005·Biochemical and Biophysical Research Communications·Satoshi KishigamiTeruhiko Wakayama
Mar 17, 2006·Molecular Reproduction and Development·Morten VejlstedPoul Maddox-Hyttel
May 27, 2006·Cell Death and Differentiation·Luigi AnastasiaBruno Venerando
Aug 22, 2006·Biochemical and Biophysical Research Communications·Eugine LeeByeong Chun Lee
Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Feikun YangValeri Zakhartchenko
Apr 13, 2007·Molecular Reproduction and Development·Kazuchika MiyoshiMitsutoshi Yoshida
May 11, 2007·Biochemical and Biophysical Research Communications·Yong Kee KimSu-Nam Kim
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Shuibing ChenPeter G Schultz
Feb 2, 2008·The Journal of Reproduction and Development·Kazuchika MiyoshiMitsutoshi Yoshida
May 17, 2008·Molecular Cancer Therapeutics·Anna Morena D'AliseRiccardo Cortese

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.