PMID: 9178355Jan 1, 1997Paper

Beneficial effects of Vero cells for developing IVF bovine eggs in two different coculture systems

Reproduction, Nutrition, Development
M C MenckY Heyman

Abstract

A Vero cell line was used for coculture of bovine in vitro fertilized eggs up to blastocyst stage in comparison with bovine oviductal epithelial cells (BOEC) in two culture systems: monolayers or microdrops. Inseminated oocytes cocultured for 7 days with Vero cells in microdrops resulted in a significantly higher blastocyst rate compared to BOEC (29.5% vs 21.1%, respectively; P < 0.01). This difference was not significant in the monolayer coculture system although the blastocyst rate tended to be higher with Vero than with BOEC monolayers (27% vs 22.3%, respectively). Interestingly, the coefficient of variation between replicates was lower in both Vero cell groups than in BOEC groups indicating that Vero cells may help reduce variability. Medium conditioned by Vero cells partly supported embryo development compared to coculture itself (14.6% vs 26.5%, respectively; P < 0.01). Blastocysts developed on Vero cells contained significantly more cells (142 +/- 39) than those developed on BOEC (88.8 +/- 32.8, P < 0.001). Viability of blastocysts developed on Vero cells was evaluated by single transfer to 26 recipient heifers. Confirmed pregnancy rate after 3 months was 58%, demonstrating their high viability.

Citations

Feb 24, 2001·Theriogenology·A M DuszewskaJ A Modliński
Apr 27, 2001·Theriogenology·P ChrenekJ P Renard
Nov 11, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·X VignonJ P Renard
May 15, 2002·Cloning and Stem Cells·Y HeymanX Vignon
Feb 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Laurence GallXavier Vignon
Apr 14, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle CôtéClaude Robert
Dec 10, 2002·Reproductive Biomedicine Online·Yves J R Ménézo, François Hérubel
Sep 21, 2006·Reproductive Biomedicine Online·Fariba MoulaviMohammad Hossein Nasr-Esfahani
Nov 23, 2006·Theriogenology·Nicolas M Orsi, Judith B Reischl
Nov 18, 2005·Developmental Biology·Séverine A DegrelleIsabelle Hue
Dec 26, 2001·Biology of Reproduction·Y HeymanJ P Renard
Oct 7, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·A Vitorino CarvalhoV Duranthon
Aug 2, 2013·Reproduction, Fertility, and Development·Hilde NelisAnn Van Soom

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