Benign mammary epithelial cells enhance the transformed phenotype of human breast cancer cells.

BMC Cancer
Joanna M PoczobuttArthur Gutierrez-Hartmann

Abstract

Recent research has yielded a wealth of data underscoring the key role of the cancer microenvironment, especially immune and stromal cells, in the progression of cancer and the development of metastases. However, the role of adjacent benign epithelial cells, which provide initial cell-cell contacts with cancer cells, in tumor progression has not been thoroughly examined. In this report we addressed the question whether benign MECs alter the transformed phenotype of human breast cancer cells. We used both in vitro and in vivo co-cultivation approaches, whereby we mixed GFP-tagged MCF-10A cells (G2B-10A), as a model of benign mammary epithelial cells (MECs), and RFP-tagged MDA-MB-231-TIAS cells (R2-T1AS), as a model of breast cancer cells. The in vitro studies showed that G2B-10A cells increase the colony formation of R2-T1AS cells in both soft agar and clonogenicity assays. Conditioned media derived from G2B-10A cells enhanced colony formation of R2-T1AS cells, whereas prior paraformaldehyde (PFA) fixation of G2B-10A cells abrogated this enhancement effect. Moreover, two other models of benign MECs, MCF-12A and HuMECs, also enhanced R2-T1AS colony growth in soft agar and clonogenicity assays. These data reveal that factors secre...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B Mintz, K Illmensee
Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C H FoxP P Roller
Mar 28, 1997·International Journal of Cancer. Journal International Du Cancer·X Dong-Le BourhisF Calvo
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·A KrtolicaJ Campisi
May 11, 2002·Breast Cancer Research and Treatment·Rober-Alain ToillonXuefen Le Bourhis
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Jayanta DebnathJoan S Brugge
Jul 9, 2003·Breast Cancer Research and Treatment·Carol A SartoriusKathryn B Horwitz
Nov 2, 2004·Nature Reviews. Cancer·Margareta M Mueller, Norbert E Fusenig
Jul 1, 2005·Breast Cancer Research : BCR·Michael SamoszukGuillaume Chorn
Oct 4, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·David R HodgeWilliam L Farrar
Jan 24, 2006·Cell Biology International·Barbara C SpinkDavid C Spink
Jan 27, 2006·The American Journal of Pathology·Shauntae M McDanielPepper Schedin
Mar 25, 2006·Nature Reviews. Cancer·Pepper Schedin
Jul 29, 2006·Cancer Detection and Prevention·Yan-gao Man, Herbert E Nieburgs
Aug 24, 2006·Breast Cancer Research and Treatment·Heike Knüpfer, Rainer Preiss
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Corinne A BoulangerGilbert H Smith
May 4, 2007·The Journal of Clinical Investigation·Wan-Wan Lin, Michael Karin
Oct 5, 2007·Nature·Antoine E KarnoubRobert A Weinberg
Dec 7, 2007·The Journal of Clinical Investigation·Pasquale SansoneMassimiliano Bonafè
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lynne-Marie PostovitMary J C Hendrix
May 6, 2008·Cancer Cell·Min HuKornelia Polyak
Sep 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brian W BoothGilbert H Smith
Oct 28, 2008·Histochemistry and Cell Biology·Marie-Aude Le Bitoux, Ivan Stamenkovic
Mar 14, 2009·Cell Cycle·Corinne A Boulanger, Gilbert H Smith
Apr 8, 2009·Journal of Mammary Gland Biology and Neoplasia·Jenean O'Brien, Pepper Schedin
Apr 22, 2009·Journal of Mammary Gland Biology and Neoplasia·Traci R LyonsVirginia F Borges

❮ Previous
Next ❯

Citations

Dec 17, 2011·International Journal of Molecular Sciences·Nurazhani Abdul RaofYubing Xie
Dec 29, 2011·Journal of Biomedicine & Biotechnology·Patricia Casbas-HernandezMelissa A Troester
Jun 2, 2017·The Open Virology Journal·S PasquereauG Herbein
Jan 10, 2019·Cell Death & Disease·Amal M El-NaggarPoul H Sorensen
Oct 14, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Guadalupe M VedoyaGabriela A Martín

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
xenograft
fluorescence microscopy
antibody
Antibody Array
X-ray
ELISA

Software Mentioned

SAS
ImageJ
STAT

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Tumorigenesis

Breast tumorigenesis involves the production or formation of tumor(s) in breast tissue. Discover the latest research on breast tumorigenesis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.