PMID: 8958793Nov 1, 1996Paper

Benign prostatic stromal cells are regulated by basic fibroblast growth factor and transforming growth factor-beta 1

The Journal of Endocrinology
A T CollinsD E Neal

Abstract

The current study was undertaken, using cultures of prostatic epithelial and stromal cells, to determine the functional interactions between androgens, basic fibroblast growth factor (FGF2) and transforming growth factor-beta 1 (TGF beta 1) and their importance in maintaining stromal homeostasis. Treatment of stromal cells with TGF beta 1 significantly increased intracellular FGF2 and FGF2 sequestered to the extracellular matrix. FGF2 was also detected in stromal conditioned medium (SCM), but at levels 70-fold less than found in cell lysates. TGF beta 1 (0.1 ng/ml) treatment caused an initial increase of 86% in secreted FGF2 levels, but high concentrations of TGF beta 1 (5 ng/ml) decreased FGF2 levels by 38%, relative to the untreated control. Further studies showed that epithelial conditioned medium (ECM), androgen-treated, stromal conditioned medium (ASCM), but not SCM were mitogenic for stromal cells. Both ECM and ASCM caused a threefold increase in DNA synthesis. FGF2 may be the mediator of these interactions, since the mitogenic effect of both ECM and ASCM was significantly reduced by the addition of anti-FGF2 neutralising antibody. We hypothesise that the lack of response of stromal cells to SCM is due to TGF beta 1 block...Continue Reading

Citations

Oct 12, 2000·The Prostate·D M Peehl, R G Sellers
Aug 27, 1998·In Vitro Cellular & Developmental Biology. Animal·D M PeehlS T Wong
Jun 8, 2002·Biochemical and Biophysical Research Communications·Hong ZhaoRajvir Dahiya
Apr 12, 2008·Molecular and Cellular Endocrinology·Paul A BerryAnne T Collins
Mar 25, 2011·The Prostate·Paul A BerryAnne T Collins
Mar 17, 2001·BJU International·Y NiuT Ma
Mar 22, 2002·Biochemical and Biophysical Research Communications·Hong ZhaoRajvir Dahiya

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