PMID: 2496105May 1, 1989Paper

Benzaldehyde lyase, a novel thiamine PPi-requiring enzyme, from Pseudomonas fluorescens biovar I

Journal of Bacteriology
B González, R Vicuña

Abstract

Pseudomonas fluorescens biovar I can grow on benzoin as the sole carbon and energy source. This ability is due to benzaldehyde lyase, a new type of enzyme that irreversibly cleaves the acyloin linkage of benzoin, producing two molecules of benzaldehyde. Benzaldehyde lyase was purified 70-fold and found to require catalytic amounts of thiamine PPi (TPP) and a divalent cation as cofactors. Optimal activity was obtained with a 1.0 mM concentration of Mn2+, Mg2+, or Ca2+. Gel permeation chromatography indicated a native molecular weight of 80,000, whereas the enzyme migrated in sodium dodecyl sulfate-containing polyacrylamide gels as a single polypeptide with a molecular weight of 53,000. Benzaldehyde lyase is highly specific; of a variety of structurally related compounds tested, only benzoin and anisoin (4,4'-dimethoxybenzoin) acted as substrates, their apparent Kms being 9.0 x 10(-3) and 3.25 x 10(-2) mM, respectively. A catalytic mechanism for the enzyme is proposed.

References

Feb 1, 1979·Journal of Bacteriology·H Grimminger, H E Umbarger
Sep 15, 1975·European Journal of Biochemistry·J M LópezH Rehbein
Jan 1, 1988·Archives of Microbiology·B GonzálezR Vicuña
Jan 1, 1969·Annual Review of Biochemistry·L O Krampitz
Jan 1, 1970·Vitamins and Hormones·J UllrichH Holzer
Sep 26, 1966·Archives of Biochemistry and Biophysics·F R Williams, L P Hager
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Dec 1, 1986·Applied and Environmental Microbiology·B GonzalezR Vicña

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Citations

Feb 19, 2009·Biotechnology Progress·Renaud J MikolajekJochen Büchs
Jun 26, 2015·Chembiochem : a European Journal of Chemical Biology·Sean PoustJustin B Siegel
Nov 24, 2005·The FEBS Journal·Tanja G MosbacherGeorg E Schulz
May 15, 2007·Chembiochem : a European Journal of Chemical Biology·Renaud MikolajekJochen Büchs
Aug 2, 2011·Biotechnology Research International·Dessy NataliaLasse Greiner
Sep 17, 2013·The FEBS Journal·Helen C HailesMartina Pohl
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Justin B SiegelDavid Baker
Apr 12, 2008·Biotechnology and Bioengineering·Michael ZavrelAntje C Spiess
Feb 9, 2019·Microbial Cell Factories·V D JägerU Krauss
Feb 12, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Lars O WiemannMarion B Ansorge-Schumacher
Jul 10, 1998·Biochimica Et Biophysica Acta·H IdingM Pohl
Oct 1, 1993·Applied and Environmental Microbiology·P Hinrichsen, R Vicuña
Oct 18, 2005·Biochimica Et Biophysica Acta·Malea M KneenMichael J McLeish
Mar 23, 2011·Chemical Reviews·Margarita BrovettoGustavo A Seoane
Jul 12, 2016·ACS Catalysis·Nina G SchmidtWolfgang Kroutil
Mar 28, 2006·Journal of Natural Products·Shuvendu Das, John P N Rosazza
Sep 25, 2021·Chembiochem : a European Journal of Chemical Biology·Martin PengKersten S Rabe

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