PMID: 8600800Aug 1, 1995Paper

Benzidine stain for the histochemical detection of hemoglobin in splinter hemorrhage (subungual hematoma) and black heel

The American Journal of Dermatopathology
J HafnerR G Panizzon

Abstract

Minor nail trauma may cause bluish discoloration of the nail, while tangential skin trauma on the heel can result in a so-called black heel. To rule out melanoma in such clinical situations, a biopsy is needed to reveal homogeneous eosinophilic masses deposited under the nail plate or within it (transepidermal elimination). Most dermatopathologists attempt to demonstrate the presence of hemoglobin in these eosinophilic masses with Prussian blue stain, which typically remains negative. In our experience, these traumatically induced blood deposits are always situated in avascular spaces, devoid of degrading phagocytes. Consequently, a histochemical stain for these deposits should be directed specifically toward hemoglobin, not hemosiderin. In the dermatopathologic literature, the various techniques to detect hemoglobin deposits in tissue sections are not well-known. We would like to emphasize benzidine stain, a highly selective and efficient method to demonstrate the presence of hemoglobin deposits in histologic sections. To date, benzidine stain has not been utilized to characterize splinter hemorrhage (subungual hematoma). Of concern, the use of benzidine in histopathology laboratories is restricted because this agent is a know...Continue Reading

Citations

Jun 8, 2017·Journal of Cutaneous Pathology·Christina WlodekDavid de Berker
Jul 22, 2008·The Australasian Journal of Dermatology·Francisco UrbinaEmilio Sudy
Nov 6, 2010·Seminars in Cutaneous Medicine and Surgery·Beth S Ruben
Nov 28, 2017·Journal of the European Academy of Dermatology and Venereology : JEADV·L R BaranL Thomas
Jan 13, 2006·Journal of the European Academy of Dermatology and Venereology : JEADV·C De La TorreT Rodríguez

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