Benzo[a]pyrene activates interleukin-6 induction and suppresses nitric oxide-induced apoptosis in rat vascular smooth muscle cells

PloS One
Huei-Ping TzengShing Hwa Liu

Abstract

Benzo[a]pyrene, a ubiquitous environmental pollutant, has been suggested to be capable of initiating and/or accelerating atherosclerosis. Accumulation of vascular smooth muscle cells (VSMCs) in vessel intima is a hallmark of atherosclerosis. Nitric oxide (NO) can suppress VSMCs proliferation and induce VSMCs apoptosis. NO plays a compensatory role in the vascular lesions to reduce proliferation and/or accelerate apoptosis of VSMCs. The aim of this study was to investigate whether benzo[a]pyrene can affect VSMCs growth and apoptosis induced by NO. Benzo[a]pyrene (1-30 μmol/L) did not affect the cell number and cell cycle distribution in VSMCs under serum deprivation condition. Sodium nitroprusside (SNP), a NO donor, decreased cell viability and induced apoptosis in VSMCs. Benzo[a]pyrene significantly suppressed SNP-induced cell viability reduction and apoptosis. VSMCs cultured in conditioned medium from cells treated with benzo[a]pyrene could also prevent SNP-induced apoptosis. Benzo[a]pyrene was capable of inducing the activation of nuclear factor (NF)-κB and phosphorylation of p38 mitogen-activated protein kinase (MAPK) in VSMCs. Both NF-κB inhibitor and p38 MAPK inhibitor significantly reversed the anti-apoptotic effect of be...Continue Reading

References

Aug 1, 1991·European Journal of Clinical Investigation·S Moncada, E A Higgs
Aug 1, 1994·The Journal of Experimental Medicine·G K HanssonE Jennische
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·H E von der LeyenV J Dzau
Jun 1, 1995·Circulation·J M IsnerJ Passeri
May 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N S KwonH S Lee
Dec 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J L HoughC Wheelock
Apr 5, 1996·Biochemical and Biophysical Research Communications·E NishioY Watanabe
Nov 1, 1996·The American Journal of Physiology·J S Beckman, W H Koppenol
Jun 1, 1997·Trends in Pharmacological Sciences·P NicoteraG Bagetta
Nov 5, 1997·The Journal of Pediatrics·E BaraldiF Zacchello
Jun 20, 1998·Circulation Research·A Haunstetter, S Izumo
Feb 19, 1999·Circulation Research·Y M KimT R Billiar
May 13, 1999·Biochimica Et Biophysica Acta·M Kelm
Nov 10, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·T TokunouA Takeshita
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anup RamachandranVictor Darley-Usmar
Jun 11, 2002·Archives of Biochemistry and Biophysics·Ming ZhaoJohn W Eaton
Jun 25, 2002·Toxicology and Applied Pharmacology·Bernhard HennigLarry W Robertson
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Dec 31, 2002·Journal of Molecular and Cellular Cardiology·Peter ZahradkaShawn Thomas
Jun 14, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hui-Ping TzengShing-Hwa Liu
Dec 18, 2003·Annals of the New York Academy of Sciences·Hyewon ParkYongsoo Park
Apr 16, 2005·Circulation Research·Felix B MehrhofClaus Scheidereit
Jun 9, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniëlle M J CurfsFrederik J van Schooten
Jan 2, 2007·The American Journal of Cardiology·Won-Young LeeElizabeth Barrett-Connor
Jan 23, 2008·Journal of Cardiovascular Pharmacology·Yong-Hae SonKoanhoi Kim
Aug 2, 2008·Toxicology and Applied Pharmacology·Elizabeth OesterlingBernhard Hennig
Jan 27, 2009·American Journal of Respiratory Cell and Molecular Biology·Aaron B Waxman, Narasaiah Kolliputi
Feb 24, 2009·Nitric Oxide : Biology and Chemistry·Muneera R KapadiaMelina R Kibbe
Apr 4, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Koteswara R ChavaGadiparthi N Rao
Apr 18, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Keita InanagaKenji Sunagawa
Sep 22, 2009·PloS One·Colleen M SnyderNavdeep S Chandel
Apr 13, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Daniel A PopowichMelina R Kibbe
Sep 30, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ching-Hao LiJaw-Jou Kang

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Citations

Aug 9, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Jia ShiFan He
Jul 7, 2020·Apoptosis : an International Journal on Programmed Cell Death·Dimitra AravaniMartin Bennett
Jul 8, 2019·Vascular Pharmacology·Huei-Ping TzengKuo-Cheng Lan

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Methods Mentioned

BETA
FCS
scraping
ELISA
flow cytometry

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