PMID: 43491Aug 1, 1979

Benzodiazepine receptors: effect of tissue preincubation at 37 degrees C

Neuroscience Letters
R C SpethH I Yamamura

Abstract

[3H]Flunitrazepam binding to rat brain homogenates was assayed in two ways: by 37-0 degrees C incubations or 0 degrees C only incubations. The affinity of brain benzodiazepine receptors for [3H]flunitrazepam was significantly greater in 37-0 degrees C incubated samples than in 0 degrees C incubated samples, while there was no change in the number of benzodiazepine receptors. The possible mechanisms for this enhanced binding affinity as well as the methodological implications for this observation are discussed.

Citations

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C Braestrup, R F Squires
Jul 27, 1978·Nature·J F TallmanD W Gallager
Aug 15, 1978·European Journal of Pharmacology·G J WastekH I Yamamura
Sep 1, 1978·The British Journal of Psychiatry : the Journal of Mental Science·C Braestrup, R F Squires
Apr 21, 1977·Nature·R F Squires, C Brastrup
Sep 1, 1978·Neuroscience Letters·W E MüllerU Wollert

Related Concepts

Anti-Anxiety Effect
Brain
Receptors, Drug
Flunitrazepam-ratiopharm
Rat Brain
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In Vitro [Publication Type]
Metazoa
Flunitrazepam
Rats, Laboratory

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