Benzodiazepines alter nucleotide and nucleoside hydrolysis in zebrafish (Danio rerio) brain

Journal of Neural Transmission
Stefani AltenhofenCarla D Bonan

Abstract

Anxiety is characterized by unpleasant bodily sensations, such as pounding heart and intense fear. The therapy involves the administration of benzodiazepine drugs. Purinergic signaling participates in the induction of several behavioral patterns and their actions are inactivated by ectonucleotidases and adenosine deaminase (ADA). Since there is evidence about the involvement of purinergic system in the actions mediated by benzodiazepines, we evaluated the effects in vitro and in vivo of administration of diazepam and midazolam on nucleoside triphosphate diphosphohydrolases, ecto-5'-nucleotidase, and ADA activities in zebrafish brain, followed by the analysis of gene expression pattern of these enzymes and adenosine receptors (A1, A2a1, A2a2, A2b). The in vitro studies demonstrated that diazepam decreased ATP (66 % for 500 µM) and ADP hydrolysis (40-54 % for 10-500 µM, respectively). Midazolam decreased ATP (16-71 % for 10-500 µM, respectively) and ADP (48-73.5 % for 250-500 µM, respectively) hydrolysis as well as the ecto-ADA activity (26-27.5 % for 10-500 µM, respectively). AMP hydrolysis was decreased in animals treated with of 0.5 and 1 mg/L midazolam (32 and 36 %, respectively). Diazepam and midazolam decreased the ecto-ADA...Continue Reading

References

Apr 1, 1979·Canadian Journal of Physiology and Pharmacology·J W Phillis
Aug 1, 1992·The Journal of Pharmacy and Pharmacology·G B KaplanD J Greenblatt
Jul 15, 1992·The Biochemical Journal·H Zimmermann
Jun 1, 1988·Pharmacology, Biochemistry, and Behavior·M HawkinsM Radulovacki
Sep 1, 1986·Analytical Biochemistry·K M ChanK D Junger
Jan 1, 1985·General Pharmacology·G Burnstock, C Kennedy
Apr 1, 1980·The Journal of Pharmacy and Pharmacology·A S BenderP H Wu
Jan 1, 1981·General Pharmacology·J W PhillisA S Bender
Jan 1, 1995·Journal of Receptor and Signal Transduction Research·M AnzeliusJ G Richards
Jul 1, 1997·Progress in Neurobiology·R FrancoC Lluis
Feb 24, 2001·Life Sciences·R A Cunha, J A Ribeiro
Aug 6, 2003·Life Sciences·Eduardo Pacheco RicoCarla Denise Bonan
Oct 7, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mario Roberto SengerCarla Denise Bonan
Jun 9, 2005·Archives of General Psychiatry·Ronald C KesslerEllen E Walters
May 12, 2007·Acta Biochimica Et Biophysica Sinica·Rongying TangDonald R Love
Jan 5, 2008·Handbook of Experimental Pharmacology·K T Olkkola, J Ahonen
Apr 12, 2008·Purinergic Signalling·Norbert Sträter
Apr 12, 2008·Purinergic Signalling·Alison Grinthal, Guido Guidotti
Jun 28, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Denis Broock RosembergDiogo Onofre Souza
Aug 1, 2009·Pharmacology, Biochemistry, and Behavior·Zachary BencanEdward D Levin
Nov 26, 2010·Archives of Disease in Childhood. Education and Practice Edition·Mark Anderson
May 17, 2011·Pharmacology, Biochemistry, and Behavior·Daiane L GebauerDiogo R Lara
May 24, 2011·Neurochemical Research·Angelo L PiatoCarla D Bonan
May 27, 2011·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Gian Luca ColussiLeonardo A Sechi
Sep 13, 2011·Brain Research Bulletin·Anna Maria SiebelCarla Denise Bonan
Sep 13, 2011·Neurotoxicology and Teratology·E P RicoC D Bonan
Oct 25, 2011·Annual Review of Pharmacology and Toxicology·Randall T Peterson, Calum A Macrae
Jan 5, 2012·The Journal of Biological Chemistry·Joseph E RittinerMark J Zylka
Sep 22, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Katiucia Marques CapiottiCarla Denise Bonan
Feb 6, 2013·ILAR Journal·Monte Matthews, Zoltán M Varga

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