PMID: 9523825Apr 2, 1998Paper

Benzodiazepines differentially inhibit phenylephrine-induced calcium oscillations in pulmonary artery smooth muscle cells

Anesthesiology
S J HongP A Murray

Abstract

Modulation of intracellular free calcium is a critical determinant of vasomotor tone. The authors investigated the effects of three benzodiazepines on alpha-adrenergic-induced oscillations in intracellular free calcium in individual pulmonary artery smooth muscle cells. Pulmonary artery smooth muscle cells were cultured from explants of canine intrapulmonary artery. Fura-2-loaded pulmonary artery smooth muscle cells were continuously superfused with phenylephrine (10 microM) at 37 degrees C on the stage of an inverted fluorescence microscope. Intracellular free calcium was measured using a dual wavelength spectrofluorometer. After establishment of steady-state intracellular free calcium oscillations induced by phenylephrine, lorazepam, diazepam, or midazolam was added to the superfusate. The amplitude and frequency of the intracellular free calcium oscillations were compared before and after addition of each agent. Resting mean +/- SEM values of intracellular free calcium were 68 +/- 8 nM. Phenylephrine stimulated dose-dependent oscillations in intracellular free calcium, which reached a peak concentration of 676 +/- 35 nM and a frequency of 1.08 +/- 0.1 transients/min. Addition of lorazepam (1 microM) inhibited (P < 0.05) the ...Continue Reading

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Citations

Feb 24, 2006·Anesthesia and Analgesia·Soon-Eun ParkYoung-Kyun Chung
Apr 9, 2005·European Journal of Anaesthesiology·A P Klockgether-RadkeG Hellige
Apr 22, 2003·European Journal of Anaesthesiology·A P Klockgether-RadkeG Hellige
Apr 22, 2003·European Journal of Anaesthesiology·N W F Linton, R A F Linton
Apr 22, 2003·European Journal of Anaesthesiology·M FattoruttoL Barvais
Apr 22, 2003·European Journal of Anaesthesiology·K HellwagnerF X Lackner
Apr 22, 2003·European Journal of Anaesthesiology·M KayaD N Kadiogullari
Apr 22, 2003·European Journal of Anaesthesiology·A Manohin, M Manohin
Apr 22, 2003·European Journal of Anaesthesiology·S GoelV K Grover
Apr 22, 2003·European Journal of Anaesthesiology·S WhiteK Henderson
Apr 22, 2003·European Journal of Anaesthesiology·K PayneG A McHugh
Apr 22, 2003·European Journal of Anaesthesiology·J DahnK Hörmann
Apr 22, 2003·European Journal of Anaesthesiology·K PayneG A McHugh

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