Benzoic Acid-Inducible Gene Expression in Mycobacteria

PloS One
Marte S DragsetMagnus Steigedal

Abstract

Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in Mycobacterium smegmatis and Mycobacterium tuberculosis, the causative agent of human tuberculosis. By making simple changes to a Gram-negative broad-host-range XylS/Pm-regulated gene expression vector, we prove that it is possible to adapt this well-studied expression system to non-Gram-negative species. With the benzoic acid-derived inducer m-toluate, we achieve a robust, time- and dose-dependent reversible induction of Pm-mediated expression in mycobacteria, with low background expression levels. XylS/Pm is thus an important addition to existing mycobacterial expression tools, especially when low basal expression is of particular importance.

References

Jun 6, 1991·Nature·C K StoverG F Hatfull
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S InouyeT Nakazawa
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H A de BoerM Vasser
Nov 1, 1995·Antimicrobial Agents and Chemotherapy·S RadulovicA F Azad
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·H KomedaS Shimizu
Jul 1, 1997·Microbiology·Tanya ParishElaine O Colston
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y YuanC E Barry
Feb 25, 1999·Journal of Applied Microbiology·R J Lambert, M Stratford
Apr 12, 2000·Journal of Immunological Methods·A Pörtner-TalianaJ P Hoeffler
Aug 10, 2000·Metabolic Engineering·H C Winther-LarsenS Valla
Dec 5, 2000·Trends in Genetics : TIG·R Schleif
Feb 22, 2001·Antimicrobial Agents and Chemotherapy·P E SilvaJ A Aínsa
May 31, 2003·Nature Reviews. Genetics·Lynn MieselTodd A Black
Mar 24, 2004·Nature Reviews. Microbiology·Douglas F Browning, Stephen J Busby
Jul 10, 2004·Cell·Sanie MnaimnehTimothy R Hughes
Feb 3, 2005·Nucleic Acids Research·Sabine EhrtDirk Schnappinger
Feb 3, 2005·Nucleic Acids Research·Marian C J BlokpoelBrian D Robertson
May 8, 2007·Journal of Bacteriology·Xinzheng V GuoDirk Schnappinger
Sep 11, 2007·Journal of Bacteriology·Daniel L MilliganKurt L Krause
Sep 20, 2008·Tuberculosis·Amit K PandeyChristopher M Sassetti
Jan 29, 2009·Nucleic Acids Research·Marcus KlotzscheDirk Schnappinger
Feb 10, 2009·Applied and Environmental Microbiology·Ingrid BakkeSvein Valla
May 12, 2009·Journal of Biotechnology·Francesca FortiDaniela Ghisotti
Apr 22, 2010·Nucleic Acids Research·Francesca BoldrinRiccardo Manganelli
Jan 1, 2009·Microbial Biotechnology·Trygve BrautasetSvein Valla
Sep 2, 2011·Future Medicinal Chemistry·Takushi KanekoKhisimuzi Mdluli
Jan 27, 2012·PloS One·Jessica C SeeligerCarolyn R Bertozzi
Mar 16, 2012·Genetics·Gregory Prelich
May 7, 2013·Bioengineered·Friederike ZwickSvein Valla

❮ Previous
Next ❯

Citations

Sep 14, 2018·Nucleic Acids Research·Ciarán L KellyAntonis Papachristodoulou

❮ Previous
Next ❯

Datasets Mentioned

BETA
KT239651
ABW35374.1

Methods Mentioned

BETA
PCR
Protein Assay
Bioscreen

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.