PMID: 2112900Jul 1, 1990Paper

Benzoic acid inhibition of the alpha, beta, and gamma isozymes of Agaricus bisporus tyrosinase

Archives of Biochemistry and Biophysics
S MenonK G Strothkamp

Abstract

Inhibition of the catecholase and cresolase reactions of the alpha, beta, and gamma isozymes of Agaricus bisporus tyrosinase by benzoic acid was investigated at 25.0 and 8.0 degrees C at pH 5.60 in air-saturated solutions. Benzoic acid is a simple competitive inhibitor of the cresolase reaction of all three isozymes. In the catecholase reaction, however, benzoic acid is a partial uncompetitive inhibitor of the alpha and beta isozymes and a simple competitive inhibitor of gamma-tyrosinase. Equilibrium dialysis experiments, conducted under identical conditions to the kinetic studies, indicate that benzoic acid can bind to the alpha and gamma isozymes in the absence of organic substrate. The dissociation constants obtained by equilibrium dialysis are in good agreement with the kinetic Ki values determined from inhibition studies. Maximum binding of benzoic acid to alpha and gamma tyrosinase, however, is significantly less than one mole per mole of active sites. A scheme in which benzoic acid binds to the oxy-form of tyrosinase is proposed to account for the kinetic and equilibrium results.

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Citations

Dec 6, 2005·Analytical and Bioanalytical Chemistry·P KoczońW Lewandowski
Apr 13, 1994·Biochimica Et Biophysica Acta·M García MorenoF García Cánovas
Oct 1, 2013·Fish & Shellfish Immunology·Cédric Le BrisFabienne Guérard
Jan 1, 1994·Critical Reviews in Food Science and Nutrition·J J NicolasS Y Aubert
Sep 25, 2015·IUBMB Life·Carmen Vanessa Ortiz-RuizFrancisco Garcia-Canovas
Mar 17, 2007·ChemMedChem·Yovani Marrero-PonceRichard Rotondo
Jul 23, 1998·Critical Reviews in Biotechnology·S RajarathnamZ Bano
Oct 7, 2005·Journal of Enzyme Inhibition and Medicinal Chemistry·N GheibiA A Moosavi-Movahedi
Dec 31, 2003·The Journal of Biological Chemistry·Armand W J W TepperGerard W Canters
Sep 18, 2002·The Journal of Biological Chemistry·Giuseppe BattainiLuigi Bubacco
Jul 29, 2004·Biochimica Et Biophysica Acta·Beata GasowskaHubert Wojtasek
May 2, 2003·Journal of Agricultural and Food Chemistry·Sung-Yum SeoNiti Sharma
Jun 21, 2001·Journal of Agricultural and Food Chemistry·P KoczońE Sobczak

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