Benzoyl peroxide increases UVA-induced plasma membrane damage and lipid oxidation in murine leukemia L1210 cells

The Journal of Investigative Dermatology
S H IbbotsonI E Kochevar

Abstract

Ultraviolet A radiation induces oxidative stress and cell damage. The purpose of this investigation was to examine whether ultraviolet A-induced cell injury was amplified by the presence of a non-ultraviolet A absorbing molecule capable of generating free radicals. Benzoyl peroxide was used as a lipid soluble potential radical-generating agent. Plasma membrane permeability assessed by trypan blue uptake was used to measure cell damage in murine leukemia L1210 cells. Cells were irradiated with a pulsed Nd/YAG laser at 355 nm using 0-160 J per cm2. The ratio of the fluence-response slope in the presence of 40 microM benzoyl peroxide to that of irradiated controls was 4.3 +/- 2.6. Benzoyl peroxide alone or benzoyl peroxide added after irradiation did not cause increased trypan blue uptake. The ratio of the fluence-response slopes in the presence of 40 microM benzoyl peroxide to that of irradiated controls was 4.7 +/- 1.4 when cells were irradiated (0-43 J per cm2) with a xenon lamp, filtered to remove wavelengths <320 nm. The increased trypan blue uptake in 355 nm-irradiated cells in the presence of benzoyl peroxide was inhibited in a concentration-dependent manner by butylated hydroxytoluene, vitamin E, and trolox, a water-solubl...Continue Reading

References

Sep 1, 1992·Photochemistry and Photobiology·M L Hu, A L Tappel
Sep 1, 1995·Free Radical Biology & Medicine·P MorliereI Tirache
Oct 1, 1993·Photochemistry and Photobiology·F GaboriauL Dubertret
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Citations

Dec 12, 2012·International Journal of Molecular Sciences·Marina T NechiforMircea Leabu
Jun 26, 1999·The Journal of Investigative Dermatology·S H IbbotsonI E Kochevar
Oct 11, 2003·Photodermatology, Photoimmunology & Photomedicine·Christelle GélisPatricia Vicendo
May 23, 2001·Photochemistry and Photobiology·Y IbukiR Goto
Jun 7, 2006·Free Radical Research·Cristina RotaAnna Iannone
Sep 19, 2009·Expert Opinion on Pharmacotherapy·Matt SagranskySteven R Feldman
Oct 30, 2004·Journal of Biomedical Materials Research. Part a·R DatarG S Schuster

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