Benzydamine inhibits osteoclast differentiation and bone resorption via down-regulation of interleukin-1 β expression

Acta Pharmaceutica Sinica. B
Han Saem SonWoojin Jeong

Abstract

Bone diseases such as osteoporosis and periodontitis are induced by excessive osteoclastic activity, which is closely associated with inflammation. Benzydamine (BA) has been used as a cytokine-suppressive or non-steroidal anti-inflammatory drug that inhibits the production of pro-inflammatory cytokines or prostaglandins. However, its role in osteoclast differentiation and function remains unknown. Here, we explored the role of BA in regulating osteoclast differentiation and elucidated the underlying mechanism. BA inhibited osteoclast differentiation and strongly suppressed interleukin-1β (IL-1β) production. BA inhibited osteoclast formation and bone resorption when added to bone marrow-derived macrophages and differentiated osteoclasts, and the inhibitory effect was reversed by IL-1β treatment. The reporter assay and the inhibitor study of IL-1β transcription suggested that BA inhibited nuclear factor-κB and activator protein-1 by regulating IκB kinase, extracellular signal regulated kinase and P38, resulting in the down-regulation of IL-1β expression. BA also promoted osteoblast differentiation. Furthermore, BA protected lipopolysaccharide- and ovariectomy-induced bone loss in mice, suggesting therapeutic potential against inf...Continue Reading

Citations

Jul 24, 2020·Journal of Cellular Physiology·You-Jin JoWoojin Jeong
Jul 14, 2020·Oxidative Medicine and Cellular Longevity·Yi-Ting ZhouJin-Wen Xu
Jan 23, 2021·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Bao-Ming TangZhuo-Ya Wang
Jul 17, 2021·Acta Biomaterialia·Xiangbing WuHongchang Lai
Jul 1, 2021·Journal of Cellular Physiology·Hyun Jin KimWoojin Jeong

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BETA
PCR
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nuclear translocation

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