Benzyl isothiocyanate alters the gene expression with cell cycle regulation and cell death in human brain glioblastoma GBM 8401 cells

Oncology Reports
Nou-Ying TangJing-Gung Chung

Abstract

Glioblastoma multiforme (GBM) is a highly malignant devastating brain tumor in adults. Benzyl isothiocyanate (BITC) is one of the isothiocyanates that have been shown to induce human cancer cell apoptosis and cell cycle arrest. Herein, the effect of BITC on cell viability and apoptotic cell death and the genetic levels of human brain glioblastoma GBM 8401 cells in vitro were investigated. We found that BITC induced cell morphological changes, decreased cell viability and the induction of cell apoptosis in GBM 8401 cells was time-dependent. cDNA microarray was used to examine the effects of BITC on GBM 8401 cells and we found that numerous genes associated with cell death and cell cycle regulation in GBM 8401 cells were altered after BITC treatment. The results show that expression of 317 genes was upregulated, and two genes were associated with DNA damage, the DNA-damage-inducible transcript 3 (DDIT3) was increased 3.66-fold and the growth arrest and DNA-damage-inducible α (GADD45A) was increased 2.34-fold. We also found that expression of 182 genes was downregulated and two genes were associated with receptor for cell responses to stimuli, the EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1) was inhibited 2....Continue Reading

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Citations

Sep 16, 2016·The American Journal of Chinese Medicine·Kuo-Ching LiuJing-Gung Chung
Nov 18, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Prabhakaran Soundararajan, Jung Sun Kim
Nov 27, 2020·International Journal of Molecular Sciences·Guomin HuangHong Zhang
May 15, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Thao Nhu DinhKooi Yeong Khaw
Jun 3, 2021·International Journal of Molecular Sciences·Annalisa PecoraroAnnapina Russo

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