Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells

Molecular Cancer Therapeutics
Sudheer K MantenaSantosh K Katiyar

Abstract

Berberine, a naturally occurring isoquinoline alkaloid, has been shown to possess anti-inflammatory and antitumor properties in some in vitro systems. Here, we report that in vitro treatment of androgen-insensitive (DU145 and PC-3) and androgen-sensitive (LNCaP) prostate cancer cells with berberine inhibited cell proliferation and induced cell death in a dose-dependent (10-100 micromol/L) and time-dependent (24-72 hours) manner. Treatment of nonneoplastic human prostate epithelial cells (PWR-1E) with berberine under identical conditions did not significantly affect their viability. The berberine-induced inhibition of proliferation of DU145, PC-3, and LNCaP cells was associated with G1-phase arrest, which in DU145 cells was associated with inhibition of expression of cyclins D1, D2, and E and cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 proteins, increased expression of the Cdk inhibitory proteins (Cip1/p21 and Kip1/p27), and enhanced binding of Cdk inhibitors to Cdk. Berberine also significantly (P < 0.05-0.001) enhanced apoptosis of DU145 and LNCaP cells with induction of a higher ratio of Bax/Bcl-2 proteins, disruption of mitochondrial membrane potential, and activation of caspase-9, caspase-3, and poly(ADP-ribose) polymer...Continue Reading

References

Sep 1, 1992·Cancer Metastasis Reviews·J A Hickman
Jan 1, 1989·Annual Review of Biochemistry·L F Liu
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Mar 1, 1995·Cancer Metastasis Reviews·M B KastanC J Leonard
Mar 9, 1995·Nature·D O Morgan
Sep 1, 1997·The Prostate·D G Tang, A T Porter
Oct 23, 1997·Journal of the American Dietetic Association·W J Craig
Nov 14, 1997·Science·W K Hong, M B Sporn
May 23, 1998·Annual Review of Immunology·D T Chao, S J Korsmeyer
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Aug 28, 1998·Science·D R Green, J C Reed
Jul 10, 1999·The Journal of Biological Chemistry·B B Wolf, D R Green
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Aug 24, 1999·Cancer Metastasis Reviews·M J PilatK J Pienta
Sep 1, 1999·The American Journal of Chinese Medicine·J G LinT F Wang
Sep 9, 1999·The American Journal of Clinical Nutrition·W J Craig
Jun 30, 2000·Current Opinion in Cell Biology·M Crompton
Oct 26, 2000·Nature·M O Hengartner
Nov 4, 2000·Cell Proliferation·M Molinari
Feb 13, 2001·Trends in Biochemical Sciences·J M Adams, S Cory
Dec 6, 2002·Cancer Metastasis Reviews·Omer Kucuk
Feb 21, 2004·CA: a Cancer Journal for Clinicians·Ahmedin JemalUNKNOWN American Cancer Society
Feb 22, 2005·Neoplasia : an International Journal for Oncology Research·Anshu M RoySantosh K Katiyar

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Citations

Nov 5, 2013·Environmental Toxicology and Pharmacology·Hong ZhengFang Ni
Sep 1, 2012·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Michael Murray
May 27, 2011·Molecular Cancer Therapeutics·Jing LiHaitao Zhang
Nov 17, 2010·PloS One·Yingying HanMing-Hui Zou
Apr 30, 2013·PloS One·Seoung Woo ShinDeokhoon Park
Apr 14, 2009·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Peng WeiQiao-Yan Zhang
Jan 19, 2008·World Journal of Gastroenterology : WJG·Myung-Ja YounRaekil Park
Feb 5, 2014·Biological & Pharmaceutical Bulletin·Shao-Long ZhuYuan-Ying Jiang
Sep 25, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Tayebeh Azam SaediFauziah Othman
Dec 3, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Paulina OlszewskaPaweł Szymański
Mar 8, 2014·BMC Complementary and Alternative Medicine·Ming ChuYue-dan Wang
Apr 14, 2015·Seminars in Cancer Biology·Stephanie C CaseyDean W Felsher
Aug 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luis Miguel Guamán OrtizAnna Ivana Scovassi
May 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert D WojtyczkaDanuta Idzik
May 15, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seoung Woo ShinDeokhoon Park
Sep 15, 2010·Expert Opinion on Investigational Drugs·Parameswara Rao VuddandaSanjay Singh
Nov 2, 2015·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Ali A AlshatwiP Antonisamy

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