Berberine ameliorates renal injury in a rat model of D-galactose-induced aging through a PTEN/Akt-dependent mechanism

Archives of Physiology and Biochemistry
Hemat El-Sayed El-HoranyDuaa Samir Helal

Abstract

This study aimed to investigate the protective effects of berberine (BBR) against D-galactose (D-gal)-induced renal aging in rats, pointing to its ability to modulate phosphatase and tensin homolog deleted on chromosome ten (PTEN)/Akt signalling, and to attenuate oxidative stress, inflammation and apoptosis. Renal aging was induced by subcutaneous injection of D-gal for six consecutive weeks along with simultaneous oral administration of BBR and compared to control rats and rats received individual doses of either drug. BBR treatment significantly reduced the serum levels of urea and creatinine, retrieved the alterations in kidney histopathology, and restored redox balance evidenced by alleviations of the level of malondialdehyde, 8-hydroxy-2'-deoxyguanosine and activating heme oxygenase-1 enzyme. Moreover, it markedly reduced the serum levels of pro-inflammatory mediators, along with down-regulation of PTEN expression, enhanced Akt activity, as well as significantly higher immunostaining of the anti-apoptotic marker (Bcl-2). These findings hold a great promise for the use of BBR as a protecting agent against renal aging.

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 9, 2007·Asian Journal of Andrology·M Talaat Abdel AzizAhmed T Abdel Aziz
Aug 5, 2009·Biological & Pharmaceutical Bulletin·Wei ZhangFeng Zhang
Mar 3, 2010·Biochemical and Biophysical Research Communications·Andrzej S TarnawskiAmrita Ahluwalia
Feb 4, 2012·American Journal of Physiology. Renal Physiology·Rongpei LanManjeri A Venkatachalam
Mar 13, 2012·Cell Metabolism·Ana Ortega-MolinaManuel Serrano
Apr 4, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ya-Yun HsuYi-Ching Lo
Jan 28, 2014·Environmental Toxicology·Chengmin WangKedi Yang
Aug 20, 2014·BioMed Research International·Quynh N DinhSophocles Chrissobolis
Sep 7, 2014·Physiological Research·N Hadzi-PetrushevM Mladenov
Nov 15, 2014·International Journal of Molecular Medicine·Izak S TaitJun Lu
Jan 23, 2015·Oxidative Medicine and Cellular Longevity·Xiaotao LiCarlos Caulin
Mar 13, 2015·Frontiers in Oncology·Michele MilellaLudovica Ciuffreda
Apr 17, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Palanivel ChitraGanapasam Sudhandiran
May 23, 2015·Biochemical and Biophysical Research Communications·Sten OrreniusBoris Zhivotovsky
Jun 10, 2015·Molecular and Cellular Therapies·Fatemeh PourrajabSeyedhossein Hekmatimoghaddam
Jan 20, 2016·Genetics and Molecular Research : GMR·J X ChenS Z Shi
Feb 19, 2016·Journal of Alzheimer's Disease : JAD·Hsin-Hua LiChih-Li Lin
Apr 5, 2016·International Journal of Molecular Sciences·Yung-Ming ChangChih-Yang Huang
Aug 17, 2016·International Journal of Molecular Sciences·Xiuli ZhangJianfei Ma
Apr 18, 2017·Experimental and Therapeutic Medicine·Yiqiang XieGuankui Du

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Citations

Jun 24, 2020·International Journal of Molecular Sciences·Ali H El-FarShaker A Mousa
Aug 17, 2021·Molecular Medicine Reports·Ruiying LiuDanfei Lou

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