Berberine induces caspase-independent cell death in colon tumor cells through activation of apoptosis-inducing factor.

PloS One
Lihong WangFang Yan

Abstract

Berberine, an isoquinoline alkaloid derived from plants, is a traditional medicine for treating bacterial diarrhea and intestinal parasite infections. Although berberine has recently been shown to suppress growth of several tumor cell lines, information regarding the effect of berberine on colon tumor growth is limited. Here, we investigated the mechanisms underlying the effects of berberine on regulating the fate of colon tumor cells, specifically the mouse immorto-Min colonic epithelial (IMCE) cells carrying the Apc(min) mutation, and of normal colon epithelial cells, namely young adult mouse colonic epithelium (YAMC) cells. Berberine decreased colon tumor colony formation in agar, and induced cell death and LDH release in a time- and concentration-dependent manner in IMCE cells. In contrast, YAMC cells were not sensitive to berberine-induced cell death. Berberine did not stimulate caspase activation, and PARP cleavage and berberine-induced cell death were not affected by a caspase inhibitor in IMCE cells. Rather, berberine stimulated a caspase-independent cell death mediator, apoptosis-inducing factor (AIF) release from mitochondria and nuclear translocation in a ROS production-dependent manner. Amelioration of berberine-sti...Continue Reading

References

Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R H WhiteheadP S Jat
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M OshimaM Taketo
Mar 1, 1996·Molecular and Cellular Biology·G M D'AbacoA W Burgess
Oct 26, 2000·Nature·M O Hengartner
Feb 24, 2001·Viral Immunology·I JordanW I Lipkin
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·M Leist, M Jäättelä
May 18, 2004·Cancer Cell·Vito TurkBoris Turk
May 18, 2004·Genes & Development·Wei-Xing ZongCraig B Thompson
Dec 14, 2004·The Journal of Biological Chemistry·Brian M PolsterDavid G Nicholls
Nov 18, 2006·Breast Cancer Research and Treatment·Hope RugoDebu Tripathy
Oct 4, 2007·Cell Cycle·Hanan BoujradSantos A Susin
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Rebecca C TaylorSeamus J Martin
Feb 16, 2008·Toxicology and Applied Pharmacology·Syed M MeeranSantosh K Katiyar
Jul 12, 2008·Phytotherapy Research : PTR·Mohsen Imanshahidi, Hossein Hosseinzadeh
Dec 26, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert H Whitehead, Pamela S Robinson
Dec 2, 2009·Journal of Cellular Biochemistry·Mee-Sun HyunWon-Hong Woo

❮ Previous
Next ❯

Citations

Dec 22, 2012·Future Medicinal Chemistry·Shijun WenPeng Huang
Apr 29, 2014·Apoptosis : an International Journal on Programmed Cell Death·Eun-Ae KimTae-Jin Lee
Jun 29, 2014·European Journal of Pharmacology·Parham Jabbarzadeh KaboliKing-Hwa Ling
Apr 14, 2015·Seminars in Cancer Biology·Stephanie C CaseyDean W Felsher
Aug 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luis Miguel Guamán OrtizAnna Ivana Scovassi
Feb 7, 2016·Fitoterapia·Chang-Shun LiuXiao-Ying Long
Feb 4, 2016·Cancer Cell International·Ichrak Riahi-ChebbiKhadija Essafi-Benkhadir
Oct 7, 2014·Biochimica Et Biophysica Acta·Ana C MoreiraVilma A Sardão
May 9, 2014·Scientific Reports·Linda BalestriniRenata Batistoni
Mar 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Betty Yuen Kwan LawVincent Kam Wai Wong
Jul 22, 2014·BioMed Research International·Luis Miguel Guamán OrtizAnna Ivana Scovassi
Jan 27, 2015·Journal of Natural Medicines·Bruna Braga DantasDemetrius Antônio Machado Araújo
Sep 4, 2014·The American Journal of Chinese Medicine·Chunqiu ChenMartin Storr
Nov 28, 2013·BMC Gastroenterology·Hailong CaoBangmao Wang
Sep 4, 2018·Expert Opinion on Drug Delivery·Jiao WuChang-Bai Liu
May 6, 2019·International Journal of Molecular Sciences·Viktor MilataLubos Cipak
Aug 2, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Dandan LiTianhui Hu
Feb 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hong-Jhih LinJin-Yi Wu
Apr 19, 2020·Medical Oncology·Jamal HallajzadehBahman Yousefi

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
flow cytometry
nuclear translocation
transfection
protein assay
electrophoresis
fluorescence microscopy

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Breast Tumorigenesis

Breast tumorigenesis involves the production or formation of tumor(s) in breast tissue. Discover the latest research on breast tumorigenesis here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.