Berberine inhibits Chlamydia pneumoniae infection-induced vascular smooth muscle cell migration through downregulating MMP3 and MMP9 via PI3K

European Journal of Pharmacology
Lu MaLijun Zhang

Abstract

The mechanisms by which Chlamydia pneumoniae infection promote vascular smooth muscle cell (VSMC) migration required in the development of atherosclerosis have not yet been fully clarified. Matrix metalloproteinases (MMPs) have important roles in VSMC migration. However, it is still unknown whether MMPs are involved in C. pneumoniae infection-induced VSMC migration. In addition, whether berberine can exert its inhibitory effects on the infection-induced VSMC migration also remains unclear. Accordingly, we investigated the effects of berberine on C. pneumoniae infection-induced VSMC migration and explored the possible mechanisms involved in this process. Herein, we found that C. pneumoniae infection could induce VSMC migration through Matrigel-coated membrane (P<0.05), which can be significantly inhibited by the broad-spectrum MMP inhibitor GM6001 (P<0.05). Our results also showed that C. pneumoniae infection upregulated both mRNA and protein expressions of MMP3 and MMP9 (P<0.05). The specific phosphoinositide 3-kinase (PI3K) inhibitor LY294002 significantly suppressed the increases in MMP3 and MMP9 protein expressions induced by C. pneumoniae infection (P<0.05). Further experiments showed that berberine significantly attenuated...Continue Reading

References

Sep 17, 2002·Current Opinion in Cell Biology·Motoharu Seiki
Sep 18, 2003·FEMS Immunology and Medical Microbiology·Jürgen RödelEberhard Straube
Aug 7, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Peter ZahradkaNatalia Yurkova
Mar 7, 2007·Expert Review of Cardiovascular Therapy·Jason Lee Johnson
Nov 10, 2011·The Journal of Microbiology·Li Jun ZhangYi Kang
Apr 23, 2013·The Journal of Pharmacy and Pharmacology·Da-Peng ChenYuan Lin
Aug 26, 2014·Nature Reviews. Molecular Cell Biology·Matthias Krause, Alexis Gautreau

❮ Previous
Next ❯

Citations

Aug 6, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenchao WangQingyong Li
Aug 21, 2019·Central Nervous System Agents in Medicinal Chemistry·Anurag K SinghGiriraj T Kulkarni
Sep 9, 2019·International Journal of Medical Microbiology : IJMM·Ningbo ZhengLijun Zhang
Apr 6, 2021·Frontiers in Pharmacology·Yun CaiKeji Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.