Berberine inhibits growth, induces G1 arrest and apoptosis in human epidermoid carcinoma A431 cells by regulating Cdki-Cdk-cyclin cascade, disruption of mitochondrial membrane potential and cleavage of caspase 3 and PARP

Carcinogenesis
Sudheer K MantenaSantosh K Katiyar

Abstract

Chemotherapeutic approach using non-toxic botanicals may be one of the strategies for the management of the skin cancers. Here we report that in vitro treatment of human epidermoid carcinoma A431 cells with berberine, a naturally occurring isoquinoline alkaloid, decreased cell viability (3-77%, P < 0.05-0.001) and induced cell death (3-51%, P < 0.01-0.001) in a dose (5-75 microM)- and time (12-72 h)-dependent manner, which was associated with an increase in G(1) arrest. G(0)/G(1) phase of the cell cycle is known to be controlled by cyclin dependent kinases (Cdk), cyclin kinase inhibitors (Cdki) and cyclins. Our western blot analysis showed that berberine-induced G(1) cell cycle arrest was mediated through the increased expression of Cdki proteins (Cip1/p21 and Kip1/p27), a simultaneous decrease in Cdk2, Cdk4, Cdk6 and cyclins D1, D2 and E and enhanced binding of Cdki-Cdk. In additional studies, treatment of A431 cells with berberine (15-75 microM) for 72 h resulted in a significant dose-dependent increase in apoptosis (31-60%, P < 0.05-0.001) than non-berberine-treated control (11.7%), which was associated with an increased expression of pro-apoptotic protein Bax, decreased expression of anti-apoptotic proteins Bcl-2 and Bcl-xl...Continue Reading

Citations

May 11, 2013·Cell and Tissue Research·Yun QianLihuang Zhang
Sep 7, 2012·Journal of Agricultural and Food Chemistry·Han-Peng KuoMing-Ching Kao
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cheol-Hee YoonYong-Soo Bae
Aug 20, 2008·The American Journal of Chinese Medicine·Wen-Yu ChengTin-Yun Ho
May 27, 2008·Blood·Xinliang MaoAaron D Schimmer
Sep 2, 2009·Biological & Pharmaceutical Bulletin·Xu SunHeyao Wang
Jul 11, 2013·World Journal of Gastroenterology : WJG·Xiao-Ling GuoQin Cao
Dec 12, 2013·BMC Complementary and Alternative Medicine·Srilatha BadaboinaByung Yeoup Chung
Nov 1, 2013·BioMed Research International·Jing J WangBarbara J S Sanderson
Sep 4, 2010·Journal of Nucleic Acids·Motilal Maiti, Gopinatha Suresh Kumar
Jun 29, 2014·European Journal of Pharmacology·Parham Jabbarzadeh KaboliKing-Hwa Ling
Sep 25, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Tayebeh Azam SaediFauziah Othman
Apr 14, 2015·Seminars in Cancer Biology·Stephanie C CaseyDean W Felsher
Aug 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luis Miguel Guamán OrtizAnna Ivana Scovassi
May 5, 2012·Biochimica Et Biophysica Acta·Inês A BarbosaPaulo J Oliveira
Mar 28, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Cheng-Chong LiYing-Cai Niu
Aug 10, 2010·European Journal of Pharmacology·Jeevitha B PatilG K Jayaprakasha
Oct 29, 2009·Fitoterapia·Yueshan Hu, Gareth E Davies

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.