Berberine prevents human nucleus pulposus cells from IL‑1β‑induced extracellular matrix degradation and apoptosis by inhibiting the NF‑κB pathway

International Journal of Molecular Medicine
Lin LuZhewei Ye

Abstract

Intervertebral disc degeneration (IDD) is widely considered to be one of the main causes of lower back pain, which is a chronic progressive disease closely related to inflammation, nucleus pulposus (NP) cell apoptosis and extracellular matrix (ECM) degradation. Berberine (BBR) is an alkaloid compound with an anti‑inflammatory effect and has been reported to exert therapeutic action in several inflammatory diseases, including osteoarthritis. Therefore, it was hypothesized that BBR may have a therapeutic effect on IDD through inhibition of the inflammatory response. The aim of the present study was to evaluate the influence of BBR on IDD in interleukin (IL)‑1β‑treated human NP cells in vitro. The results showed that BBR attenuated the upregulation of ECM‑catabolic factors [matrix metalloproteinase (MMP)‑3, MMP‑13, a disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)‑4 and ADAMTS‑5], and the downregulation of ECM‑anabolic factors (type II collagen and aggrecan) following stimulation of the human NP cells with IL‑1β. Treatment with BBR also protected human NP cells from IL‑1β‑induced apoptosis, as determined by western blotting and flow cytometry. Mechanistically, the IL‑1β‑stimulated degradation of IκBα, and the p...Continue Reading

Citations

May 11, 2021·Oxidative Medicine and Cellular Longevity·Xi LuoJiangang Shi
Aug 17, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Hai-Wei ChenXue-Wen Kang

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Methods Mentioned

BETA
Protein Assay
electrophoresis
flow cytometry
nuclear translocation

Software Mentioned

SPSS
BandScan

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis