Berberine prevents primary peritoneal adhesion and adhesion reformation by directly inhibiting TIMP-1

Acta Pharmaceutica Sinica. B
Xin LiuBaofeng Yang

Abstract

Peritoneal adhesions are fibrous tissues that tether organs to one another or to the peritoneal wall and represent the major cause of postsurgical morbidity. Enterolysis at repeat surgeries induces adhesion reformation that is more difficult to prevent than primary adhesion. Here we studied the preventive effects of different approaches of berberine treatment for primary adhesion, and its effects on adhesion reformation compared to Interceed. We found the primary adhesion was remarkably prevented by berberine through intraperitoneal injection 30 min before abrasive surgery (pre-berberine) or direct addition into injured cecum immediately after the surgery (inter-berberine). Rats with adhesion reformation had a more deteriorative collagen accumulation and tissue injury in abrasive sites than rats with primary adhesion. The dysregulated TIMP-1/MMP balance was observed in patients after surgery, as well as adhesion tissues from primary adhesion or adhesion reformation rats. Inter-berberine treatment had a better effect for adhesion reformation prevention than Interceed. Berberine promoted the activation of MMP-3 and MMP-8 by directly blocking TIMP-1 activation core, which was reversed by TIMP-1 overexpression in fibroblasts. In co...Continue Reading

Citations

May 27, 2021·Acta Pharmacologica Sinica·Hui-Min LiBao-Feng Yang
Aug 28, 2021·Biomedicines·Ali Fatehi HassanabadPaul W M Fedak

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Methods Mentioned

BETA
electrophoresis
PCR
surface
biosensor
chip
chips
dissection
ELISA
transmission electron microscopy
nuclear magnetic resonance

Software Mentioned

SYBYL
BOSTER
SPSS
OpenSPR
AMBER
GraphPad Prism
TraceDrawer
Interceed

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