Berberine reduces fibronectin and collagen accumulation in rat glomerular mesangial cells cultured under high glucose condition.

Molecular and Cellular Biochemistry
Weihua LiuPeiqing Liu

Abstract

Diabetic nephropathy, one of the microvascular complications of diabetes mellitus, is a leading cause of end-stage renal disease. Berberine is one of the main constituents of Coptidis Rhizoma and Cortex Phellodendri. In this study, we investigated the effects of berberine on fibronectin and collagen production, and explored the role of p38MAPK signaling pathway in rat glomerular mesangial cells cultured under high glucose condition. Six groups were divided according to the different experimental conditions: (1) Normal glucose group (NG); (2) Mannitol group (Mannitol); (3) High glucose group (HG); (4) SB203580 treatment group (HG + SB203580); (5) Berberine low dosage group (HG + BBR 30 microM); (6) Berberine high dosage group (HG + BBR 90 microM). Cell proliferation and collagen synthesis were measured by MTT and (3)H-proline incorporation assay, respectively. The phospho-p38MAPK, phospho-cAMP response element binding protein (CREB) and fibronectin were detected by western blot analysis. Fibronectin protein expression and collagen synthesis were significantly increased in HG-treated group compared with normal glucose group (P < 0.05). In SB203580 treatment group and two groups of berberine, protein expression of fibronectin and ...Continue Reading

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Citations

Jul 12, 2012·Expert Opinion on Biological Therapy·Giuseppe DerosaArrigo F G Cicero
Jun 23, 2018·Chinese Medicine·Jin WangQin-Wan Huang
Aug 17, 2016·International Journal of Molecular Sciences·Xiuli ZhangJianfei Ma
Feb 6, 2018·Experimental and Therapeutic Medicine·Hong-Cai WangJian-Min Zhang
Apr 17, 2015·BMC Complementary and Alternative Medicine·Jung Joo YoonHo Sub Lee
Apr 16, 2021·Frontiers in Pharmacology·Mingming ZhaoYu Zhang

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