Berberine reduces Toll-like receptor-mediated macrophage migration by suppression of Src enhancement

European Journal of Pharmacology
Wei Erh ChengTzeng-Horng Leu

Abstract

Berberine is an isoquinoline with anti-inflammatory activity. We previously demonstrated that there was a loop of signal amplification between nuclear factor kappa B and Src for macrophage mobility triggered by the engagement of Toll-like receptors (TLRs). The simultaneous suppression of lipopolysaccharide (LPS)-mediated upregulation of inducible nitric oxide synthase, cyclooxygenase 2, and cell mobility in berberine-treated macrophages suggested Src might be a target of berberine. Indeed, th reduced migration, greatly suppressed Src induction in both protein and RNA transcript by berberine were observed in macrophages exposed to LPS, peptidoglycan, polyinosinic-polycytidylic acid, and CpG-oligodeoxynucleotides. In addition to Src induction, berberine also inhibited LPS-mediated Src activation in Src overexpressing macrophages and S-nitroso-N-acetylpenicillamine (a nitric oxide donor) could partly restore it. Moreover, berberine suppressed Src activity in fibronectin-stimulated macrophages and in v-Src transformed cells. These results implied that by effectively reducing Src expression and activity, berberine inhibited TLR-mediated cell motility in macrophages.

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Citations

Jul 8, 2016·Nature Reviews. Cardiology·Joe W E Moss, Dipak P Ramji
Nov 5, 2016·Phytotherapy Research : PTR·Mohsen Imenshahidi, Hossein Hosseinzadeh
May 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Graziano RiccioniNicolantonio D'Orazio
Jan 8, 2020·International Journal of Molecular Sciences·Chien-Chung YangChuen-Mao Yang
Sep 15, 2018·International Journal of Molecular Sciences·Chung-Yi ChenChi-Ming Liu
Jul 22, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Wei-Lun LiuHeng-Liang Lin

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