Berberine reinforces Sertoli cells niche and accelerates spermatogonial stem cells renewal in experimentally-induced varicocele condition in rats

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Hamid RashtbariHamed Najaran

Abstract

Varicocele is present in 10-20% of the male infertile population. Present study was done to demonstrate the reinforcing effect of berberine (BBR), as an antioxidant and anti-inflammatory agent, on Sertoli cells-related niche and spermatogonial stem cells (SSCs) self-renewal in experimentally-induced VCL condition. 50 mature male Wistar rats were divided into control, control-sham, non-treated VCL-induced, 50 mg kg-1and 100 mg kg-1BBR-treated VCL-induced groups. The Leydig and Sertoli cells distribution and Leydig cells steroidogenic activity, expression of glial cell line-derived neurotrophic factor (GDNF), proto-oncogene Rearranged during Transfection (c-RET) receptor, Ets variant gene 5 (Etv5) and B-cell chronic lymphocytic leukemia (CLL)/lymphoma 6, member B (Bcl-6b) at mRNA and protein levels were analyzed. The mRNA integrity and DNA fragmentation were assessed. Finally, the serum levels of testosterone, inhibin B and testicular total antioxidant capacity, total thiol molecules, catalase, and malondialdehyde were evaluated. Observations revealed that, the BBR significantly enhanced VCL-reduced Leydig and Sertoli cells population, maintained Leydig-Sertoli cells network, enhanced GDNF, c-RET Etv5 and Bcl6b expression, up-reg...Continue Reading

Citations

Nov 24, 2021·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Sara MoradpourMohammadreza Bigdeli

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