Berberine suppresses TPA-induced fibronectin expression through the inhibition of VEGF secretion in breast cancer cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Sangmin KimSeok Jin Nam

Abstract

Berberine (BBR) is an isoquinoline alkaloid and is beneficial for the anticancer effect on a variety of human tumor cells. However, BBR's anti-angiogenesis property and its clinical potential as an inhibitor of tumor angiogenesis in breast cancer cells have not been fully elucidated. Here, we investigated the effect of BBR on TPA-induced VEGF and fibronectin (FN) as well as VEGF-induced FN in breast cancer cells. The secretion of VEGF protein was detected by ELISA. Fibronectin mRNA and protein expression was analyzed by Real-Time PCR and western blotting, respectively. The overexpressions of CA-MEK, and CA-Akt were examined by adenovirus system. Our results showed that TPA, a tumor promoter, significantly increased the level of VEGF and FN expression in both MCF7 and T47D breast cancer cells. On the other hand, TPA-induced VEGF and FN expression was suppressed by LY294002, a PI-3K inhibitor. In contrast, the level of FN expression also significantly increased by constitutively active (CA)-AKT overexpression. We also found that TPA-induced VEGF and FN expression was decreased by BBR treatment. Finally, our results showed that VEGF augmented the expression of FN whereas VEGF-induced FN expression was decreased by BBR treatment. T...Continue Reading

Citations

Apr 10, 2015·International Journal of Molecular Sciences·Eun Ju ShinJin-Taek Hwang
Jun 5, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Junyi HeXinsheng Huang
Mar 5, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lenka VarinskaPeter Kruzliak
Sep 25, 2017·Chemical Biology & Drug Design·Fatemeh Mohammadipanah, Fatemeh Salimi
Dec 12, 2018·Anti-cancer Agents in Medicinal Chemistry·Anna BelanovaPeter Zolotukhin
Apr 27, 2020·Frontiers of Medicine·Danyang SongDaiming Fan
Jun 11, 2019·Oxidative Medicine and Cellular Longevity·Masoud AlasvandIlaria Peluso
Jul 4, 2020·Signal Transduction and Targeted Therapy·Zhe ZhangCanhua Huang
Nov 2, 2020·Molecular Pharmacology·Kanchan VishnoiBasabi Rana

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