Berberine treatment attenuates the palmitate-mediated inhibition of glucose uptake and consumption through increased 1,2,3-triacyl-sn-glycerol synthesis and accumulation in H9c2 cardiomyocytes

Biochimica Et Biophysica Acta
Wenguang ChangGrant M Hatch

Abstract

Dysfunction of lipid metabolism and accumulation of 1,2-diacyl-sn-glycerol (DAG) may be a key factor in the development of insulin resistance in type 2 diabetes. Berberine (BBR) is an isoquinoline alkaloid extract that has shown promise as a hypoglycemic agent in the management of diabetes in animal and human studies. However, its mechanism of action is not well understood. To determine the effect of BBR on lipid synthesis and its relationship to insulin resistance in H9c2 cardiomyocytes, we measured neutral lipid and phospholipid synthesis and their relationship to glucose uptake. Compared with controls, BBR treatment stimulated 2-[1,2-(3)H(N)]deoxy-D-glucose uptake and consumption in palmitate-mediated insulin resistant H9c2 cells. The mechanism was though an increase in protein kinase B (AKT) activity and GLUT-4 glucose transporter expression. DAG accumulated in palmitate-mediated insulin resistant H9c2 cells and treatment with BBR reduced this DAG accumulation and increased accumulation of 1,2,3-triacyl-sn-glycerol (TAG) compared to controls. Treatment of palmitate-mediated insulin resistant H9c2 cells with BBR increased [1,3-(3)H]glycerol and [1-(14)C]glucose incorporation into TAG and reduced their incorporation into DAG ...Continue Reading

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Citations

Mar 15, 2016·Biochimica Et Biophysica Acta·Vernon W DolinskyGrant M Hatch
May 18, 2017·Journal of Cellular Biochemistry·Christelle ViglinoChristophe Montessuit
Nov 8, 2018·Journal of Cellular and Molecular Medicine·Wenguang ChangMan Wang
May 2, 2018·Current Medicinal Chemistry·Maria-Ioanna ChristodoulouMaria Halabalaki
Apr 30, 2020·American Journal of Physiology. Cell Physiology·Jake S RussellJohn P Headrick
Jul 31, 2020·Medicinal Research Reviews·Xiao-Fei ShangKuo-Hsiung Lee
Mar 7, 2020·Frontiers in Pharmacology·Tarun BelwalJavier Echeverría
Sep 3, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yunfeng HanXianrong Lai

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