Best practices for application of attachment cells to in vitro micronucleus assessment by flow cytometry

Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Jeffrey C BemisAndreas Sutter

Abstract

This work seeks to provide users with guidance on cell culture, treatment, processing and analytical conditions for achieving optimal performance of the in vitro micronucleus assay using the In Vitro MicroFlow(®) method. Experimental data are provided to support the advice described. The information provided covers specific topics or issues that are identified as critical to the methodology and thus is meant to work with instruction manuals, published papers and other references, and not as a replacement for these documents. The content is divided into several sections. Cell culture and treatment describes conditions for routine maintenance of cells as well as treatment with test articles. Preparation and processing of samples details steps found to be critical in execution of the procedure. Instrument parameters and analysis covers set-up of the flow cytometer and evaluation of the samples. General assay considerations and interpretation of results describes examination of data in terms of assay validity, viability and genotoxicity assessment. The goal is to educate users and enable them to design, conduct and interpret flow cytometric in vitro micronucleus (MN) studies. Readers should obtain an understanding of specific cell ...Continue Reading

References

Oct 22, 2003·Journal of Biomolecular Screening·Betina Kerstin LundholtLen Pagliaro
Feb 10, 2010·International Journal of Cancer. Journal International Du Cancer·Amanda Capes-DavisR Ian Freshney
Oct 12, 2010·Mutation Research·Makoto HayashiVeronique Thybaud
Oct 22, 2010·Environmental and Molecular Mutagenesis·John NicoletteEric Blomme
Mar 19, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Paul FowlerStefan Pfuhler
Oct 4, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·E LorgeJ Y Tanir

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