PMID: 40530Jan 1, 1979

Beta-1-3-glucanase and dimorphism in Paracoccidioides brasiliensis

Antonie van Leeuwenhoek
A Flores-CarreónG San Blas

Abstract

Mycelial and yeast forms of P. brasiliensis were tested for several glucohydrolases. In addition to high levels of beta-glucanases, low amounts of alpha-glucanase, chitinase and maltase were found. Tests for invertase, amylase and lactase were negative. The levels of beta-1,3-glucanase were higher in the mycelial form. The shift to the mycelial phase correlated with an increase in the levels of beta-1,3-glucanase. The enzyme was present in the cytoplasm, cell wall and culture medium. The extracellular enzyme was purified 42 fold by ammonium sulphate precipitation and gel filtration. Maximal activity was obtained at 60 degrees C and pH of 5.0 (acetate buffer or pH 6.0 (phosphate buffer). Its Km was 0.205 mg/ml. The cell wall-bound enzyme showed a higher temperature optimum. Optimum pH and Km were also slightly different. Following treatment of the cell walls with chitinase, beta-1,3-glucanase was released into the medium.

References

Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·F San-BlasF Gil
Jan 12, 2013·Journal of Proteomics·José L Martínez-SalgadoAna P Barba de la Rosa
Feb 21, 2006·FEMS Immunology and Medical Microbiology·Sheyla M R C BonfimMaristela Pereira
Apr 19, 2012·Memórias do Instituto Oswaldo Cruz·Lidiane Aparecida da Penha SantanaMaristela Pereira

Citations

Jan 1, 1975·Journal of Bacteriology·Y Polacheck, R F Rosengerger
Jan 1, 1972·Archiv für Mikrobiologie·M CortatA Wiemken
Jan 1, 1974·Annual Review of Microbiology·P A Srere, K Mosbach
Apr 1, 1972·Journal of Bacteriology·F KanetsunaY Yamamura
Jul 13, 1972·Biochimica Et Biophysica Acta·V Z Holten, S BARTNICKI-GARCIA
Mar 1, 1970·Journal of Bacteriology·F Kanetsuna, L M Carbonell
Nov 24, 1970·Biochimica Et Biophysica Acta·A Flores-CarreonJ Ruiz-Herrera
Sep 1, 1968·The Biochemical Journal·A T Abd-el-Al, H J Phaff
Mar 1, 1969·Journal of Bacteriology·F KanetsunaJ Rodriguez
Apr 1, 1959·Canadian Journal of Microbiology·E T REESE, M MANDELS
Oct 1, 1962·Journal of Bacteriology·S BARTNICKI-GARCIA, W J NICKERSON
Nov 5, 1962·Biochimica Et Biophysica Acta·S BARTNICKI-GARCIA, W J NICKERSON
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

Related Concepts

Paracoccidioides brasiliensis
Phosphate buffers
Lysosomal alpha-Glucosidase
Phoma brasiliensis
Philyra brasiliensis
Extracellular
Parastacus brasiliensis
Filamentous fungus
Psychotria brasiliensis
Beta-1,3-glucanase

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.