Abstract
Integrins of the epidermis have been implicated both in intercellular adhesion and in cell-substratum adhesion. In the present study the role of alpha 2 beta 1 and alpha 3 beta 1 integrins has been evaluated further using human keratinocyte culture. alpha 3 beta 1 but not alpha 2 beta 1 strongly colocalizes with talin in adhesion plaques, consistent with a role for the former in adhesion to endogenous matrix. Upon elevation of the extracellular Ca2+ concentration from 30 microM to 1.0 mM, which is known to induce the organization of intercellular junctions, all three integrin subunits redistribute to concentrate along the cell-cell borders, but alpha 3 redistributes more slowly. Blocking antibody to E-cadherin, which has previously been shown to delay the establishment of cell-cell adhesion upon Ca2+ elevation, delays the redistribution of alpha 2 beta 1 and alpha 3 beta 1 integrins. Elevation of the Ca2+ concentration also induces a rapid morphological change in the keratinocytes and organization of the culture into colonies with tight cell-cell connections. Blocking antibodies to beta 1 or to alpha 3, but not to alpha 2, delays this morphological change and the organization into colonies; however, the effect is much more pron...Continue Reading
Citations
Jul 1, 1996·Cell Adhesion and Communication·J B WeitzmanP Brodt
May 10, 2012·Cell Adhesion & Migration·Carolina Epifano, Mirna Perez-Moreno
Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M A Stepp, L Zhu
Oct 31, 2000·Microscopy Research and Technique·A M Belkin, M A Stepp
Apr 30, 2002·Cell Biochemistry and Function·Ronan O'LearyEdward J Wood
Dec 8, 2004·The Journal of Biological Chemistry·Edith HintermannVito Quaranta
Mar 1, 1996·Oral Diseases·H LarjavaV J Uitto
Jan 28, 2003·The Journal of Biological Chemistry·Alisa VespaLina Dagnino
Feb 13, 2001·Experimental Cell Research·J D Whittard, S K Akiyama
Dec 6, 1997·Current Opinion in Genetics & Development·E FuchsQ C Yu
Jul 4, 2003·The Journal of Investigative Dermatology·James R McMillanHiroshi Shimizu
Jun 1, 1997·The Journal of Investigative Dermatology·L T Kim, K M Yamada
Oct 19, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Gustavo Pina GodoyLélia Maria Guedes Queiroz