beta 3-Adrenergic agonist induces a functionally active uncoupling protein in fat and slow-twitch muscle fibers

The American Journal of Physiology
T YoshidaM Saito

Abstract

The mitochondrial uncoupling protein (UCP) has usually been found only in brown adipose tissue. We recently observed that a chronic administration of the beta 3-adrenergic agonist CL-316,243 (CL) induced the ectopic expression of UCP in white fat and skeletal muscle in genetic obese yellow KK mice. The aim of the present study was to examine whether UCP could be induced in nongenetic obese animals produced by neonatal injections of monosodium L-glutamate (MSG). The daily subcutaneous injection of CL (0.1 mg/kg) to MSG-induced obese mice for 2 wk caused significant reductions of body weight (15%) and white fat pad weight (58%). Northern and Western blot analyses showed that CL induced significant expressions of UCP in the white fat and muscle, as well as in brown fat. Immunohistochemical observations revealed that the UCP stains in white fat were localized on multilocular cells and that those in muscle were localized on slow-twitch fibers rich in mitochondria. Immunoelectron microscopy confirmed the mitochondrial localization of UCP in the myocytes. The guanosine 5'-diphosphate (GDP) binding to mitochondria in brown fat doubled after the CL treatment. Moreover, significant GDP binding was detected in the white fat and muscle of ...Continue Reading

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·O ChampignyB R Holloway
Sep 8, 1989·Science·L J EmorineA D Strosberg
Jan 1, 1989·Progress in Lipid Research·J Himms-Hagen
Apr 1, 1989·Journal of the Neurological Sciences·M Ecob-PrinceW Brown
Jun 1, 1989·Journal of the Neurological Sciences·M Ecob-PrinceW Brown
Aug 1, 1987·Endocrinologia Japonica·H NishiokaM Kondo
Nov 1, 1984·Metabolism: Clinical and Experimental·T YoshidaM Kondo
Nov 1, 1993·Medicinal Research Reviews·J R Arch, A J Kaumann
Aug 1, 1996·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·T UmekawaM Kondo

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Citations

Aug 16, 2000·American Journal of Physiology. Cell Physiology·J Himms-HagenS Cinti
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M RossmeislJ Kopecký
Aug 11, 2005·Pflügers Archiv : European journal of physiology·Luiz Carlos C NavegantesIsis C Kettelhut
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard

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