Beta 3 adrenoreceptors protect from hypertrophic remodelling through AMP-activated protein kinase and autophagy.

ESC Heart Failure
Emilie Dubois-DeruyJean-Luc Balligand

Abstract

The abundance of beta 3-adrenergic receptors (β3-ARs) is upregulated in diseased human myocardium. We previously showed that cardiac-specific expression of β3-AR inhibits the hypertrophic response to neurohormonal stimulation. Here, we further analysed signalling pathways involved in the anti-hypertrophic effect of β3-AR. In vitro hypertrophic responses to phenylephrine (PE) were analysed in neonatal rat ventricular myocytes (NRVM) infected with a recombinant adenovirus expressing the human β3-AR (AdVhβ3). We confirmed results in mice with cardiomyocyte-specific moderate expression of human β3-AR (β3-TG) and wild-type (WT) littermates submitted to thoracic transverse aortic constriction (TAC) for 9 weeks. We observed a colocalization of β3-AR with the AMP-activated protein kinase (AMPK) both in neonatal rat and in adult mouse cardiomyocytes. Treatment of NRVM with PE induced hypertrophy and a decrease in phosphorylation of Thr172-AMPK (/2, P = 0.0487) and phosphorylation of Ser79-acetyl-CoA carboxylase (ACC) (/2.6, P = 0.0317), inducing an increase in phosphorylated Ser235/236 S6 protein (×2.5, P = 0.0367) known to be involved in protein synthesis. These effects were reproduced by TAC in WT mice but restored to basal levels in ...Continue Reading

References

Dec 1, 1987·Journal of Molecular and Cellular Cardiology·U PfeiferJ Dämmrich
Jan 1, 1983·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·J Dämmrich, U Pfeifer
Nov 11, 1999·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·P D ChamberlainG J Murphy
Dec 12, 2001·Journal of Molecular and Cellular Cardiology·S MoniotteJ L Balligand
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Jun 20, 2008·The Journal of Biological Chemistry·Anita Y M ChanJason R B Dyck
Jul 22, 2008·Journal of Molecular and Cellular Cardiology·Biao LeiWilliam C Stanley
Aug 19, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Rumi MaruyamaHisayoshi Fujiwara
Sep 25, 2008·Biochemical and Biophysical Research Communications·Elham ZarrinpashnehLuc Bertrand
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Benoit ViolletLuc Bertrand
Oct 12, 2010·Advances in Pharmacology·Chantal Dessy, Jean-Luc Balligand
Nov 15, 2011·Cell·Noboru Mizushima, Masaaki Komatsu
Jul 11, 2014·Archives of Biochemistry and Biophysics·Yanh LiYug Dong
Aug 17, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Magali BalteauChristophe Beauloye
Jan 8, 2015·Cell Metabolism·Aaron M CypessGerald M Kolodny
May 14, 2016·Circulation Research·Akihiro ShirakabeJunichi Sadoshima
Jan 29, 2017·Journal of Magnetic Resonance Imaging : JMRI·Bas T FranssensFrank L J Visseren
Aug 15, 2017·Nature Reviews. Nephrology·Pallavi Bhargava, Rick G Schnellmann
Feb 2, 2018·Nature Reviews. Cardiology·Charlotte FarahJean-Luc Balligand

❮ Previous
Next ❯

Citations

Dec 6, 2020·Cells·Lauriane Y M MichelJean-Luc Balligand
Mar 28, 2021·Cardiovascular Research·Jean-Luc Balligand, Luc Bertrand
Jun 3, 2021·International Journal of Molecular Sciences·Dianne M Perez
Sep 1, 2021·European Heart Journal·Jean-Luc Balligand, Lauriane Y M Michel

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
electrophoresis
proximity ligation
transgenic
proximity ligation assay
biopsies
Assay

Software Mentioned

AxioVision
Axiovison
Zeiss

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

Expert Opinion on Therapeutic Patents
Maria Grazia Perrone, Antonio Scilimati
Progress in Molecular Biology and Translational Science
Li-Kun Yang, Ya-Xiong Tao
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Yixi LiuWeimin Li
© 2021 Meta ULC. All rights reserved