PMID: 19912853Apr 1, 1992Paper

beta-Actin and beta-Tubulin are components of a heterogeneous mRNA population present in the squid giant axon

Molecular and Cellular Neurosciences
B B KaplanA Giuditta

Abstract

Previously, we have reported that the squid giant axon contains a heterogeneous population of polyadenylated mRNAs, as well as biologically active polyribosomes. To define the composition of this unique mRNA population, cDNA libraries were constructed to RNA obtained from the axoplasm of the squid giant axon and the parental cell bodies located in the giant fiber lobe. Here, we report that the giant axon contains mRNAs encoding beta-actin and beta-tubulin. The axonal location of these mRNA species was confirmed by in situ hybridization histochemistry, and their presence in the axoplasmic polyribosome fraction was demonstrated by polymerase chain reaction methodology. Taken together, these findings establish the identity of two relatively abundant members of the axonal mRNA population and suggest that key elements of the cytoskeleton are synthesized de novo in the squid giant axon.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1979·The Biochemical Journal·B B KaplanA E Gioio
May 1, 1977·Journal of Neurobiology·M M Black, R J Lasek
Jan 1, 1991·Journal of Neuroscience Research·A GiudittaB B Kaplan
May 1, 1986·Journal of Neurochemistry·C P CapanoB B Kaplan
Feb 7, 1985·Journal of Theoretical Biology·J Alvarez, J C Torres
Sep 1, 1987·Journal of Neurochemistry·C P CapanoB B Kaplan
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Nakajima-IijimaT Kakunaga
Aug 8, 1973·Nature: New Biology·R J LasekR Nordlander
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R J LasekR J Przybylski
Jan 15, 1974·The Journal of Comparative Neurology·R P Skoff, V Hamburger
Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·J Zelená
Apr 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A GiudittaM Brzin
Jan 1, 1969·Journal of Neurochemistry·A Edström, J Sjöstrand
Dec 1, 1982·The Journal of Cell Biology·P Cancalon
Mar 25, 1983·Nucleic Acids Research·U NudelD Yaffe
Dec 24, 1984·Brain Research·M Tytell, R J Lasek
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E KrauhsH Ponstingl
Aug 1, 1982·Journal of Neurochemistry·E Koenig, P Adams
Jun 1, 1980·Journal of Neurochemistry·A GiudittaG Lazzarini
Jan 1, 1989·The European Journal of Neuroscience·E R LunnS Gordon

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Citations

Sep 26, 2000·Journal of Neuroscience Research·J R Sotelo-SilveiraJ R Sotelo
May 1, 1994·The Histochemical Journal·J Van Minnen
Jul 10, 2007·Cellular and Molecular Neurobiology·Mi HilleforsBarry B Kaplan
Jul 20, 2002·Trends in Neurosciences·Antonio GiudittaEdward Koenig
Mar 2, 1999·Neuroscience·E Koenig, A Giuditta
Jun 18, 2002·Journal of Neurochemistry·C R JiménezJ van Minnen
Sep 1, 1993·Journal of Neurochemistry·M CrispinoA Giuditta
Jul 9, 2004·Annals of the New York Academy of Sciences·Barry B KaplanAnthony E Gioio
Dec 17, 2009·Progress in Neurobiology·Annica B Dahlstrom
Mar 31, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Aron KosArmaz Aschrafi
Jan 20, 2007·Progress in Neurobiology·Ferdinando RossiLuigi Corvetti
Jun 8, 2001·Journal of Neuroscience Research·A E GioioB B Kaplan
Sep 3, 2013·Developmental Neurobiology·José Roberto SoteloJohn A Mercer
Oct 20, 2018·Molecular Omics·Indrek Koppel, Mike Fainzilber
Apr 9, 2008·Physiological Reviews·Antonio GiudittaMarianna Crispino
May 22, 2016·Cellular and Molecular Neurobiology·Harold GainerHarish C Pant
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Hosung Jung, Christine E Holt
Mar 13, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Andreia Filipa Rodrigues Batista, Ulrich Hengst
Jun 20, 2003·The European Journal of Neuroscience·A A BattagliaA de Santis
Mar 29, 2020·PeerJ·Alexander P YoungRussell C Wyeth
Apr 13, 2021·Progress in Neurobiology·Carla Perrone-CapanoMarianna Crispino
Jul 13, 2021·Frontiers in Cellular Neuroscience·Andres Di PaoloJosé Roberto Sotelo-Silveira

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