Beta-actin is required for mitochondria clustering and ROS generation in TNF-induced, caspase-independent cell death

Journal of Cell Science
Jinquan LiJiahuai Han

Abstract

Tumor necrosis factor (TNF)-alpha induces caspase-independent cell death in the fibrosarcoma cell line L929. This cell death has a necrotic phenotype and is dependent on production of reactive oxygen species (ROS) in the mitochondria. To identify genes involved in this TNF-induced, ROS-dependent cell death pathway, we utilized retrovirus insertion-mediated random mutagenesis to generate TNF-resistant L929 cell lines and we subsequently identified genes whose mutations are responsible for the TNF-resistant phenotype. In one such resistant line, beta-actin was disrupted by viral insertion, and subsequent reconstitution of beta-actin expression levels in the mutant line Actin(mut) restored its sensitivity to TNF. Resistance to TNF in Actin(mut) cells is signal specific since the sensitivity to other death stimuli is either unchanged or even increased. Comparable NF-kappaB activation and p38 phosphorylation in TNF-treated wild-type and Actin(mut) cells also indicates that reduced expression of actin only selectively blocked some of the TNF-induced cellular changes. Actin cleavage involved in apoptosis does not occur in TNF-treated L929 cell death, as in HeLa cells. Consistent over-expression of a caspase-cleaved product, a 15 kDa a...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Nov 8, 1985·Science·L J Old
Jan 1, 1988·Annual Review of Biochemistry·B Beutler, A Cerami
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Feb 17, 1995·The Journal of Biological Chemistry·M Tewari, V M Dixit
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·V GoossensW Fiers
Apr 26, 1995·Biochemical and Biophysical Research Communications·I MelamedE W Gelfand
Sep 10, 1993·Cell·L A TartagliaD V Goeddel
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C KayalarD E Bredesen
Feb 1, 1996·Current Opinion in Genetics & Development·M Tewari, V M Dixit
Jun 6, 1998·The Journal of Experimental Medicine·D VercammenP Vandenabeele
Aug 28, 1998·Science·D R Green, J C Reed
Feb 6, 1999·The Journal of Biological Chemistry·S C Posey, B E Bierer
Apr 21, 1999·Current Opinion in Cell Biology·H Li, J Yuan
Jun 29, 1999·Cellular Immunology·I Melamed, E W Gelfand
Aug 25, 1999·The Journal of Cell Biology·J C MillsR N Pittman
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·G S Salvesen, V M Dixit
Dec 28, 1999·BioFactors·V GoossensJ Grooten
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Apr 25, 2000·Experimental Cell Research·A F AreG P Pinaev
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·S R WhiteD R Dorscheid
Nov 20, 2001·Molecular and Cellular Biology·K OnoJ Han
Jan 10, 2002·Biochemical and Biophysical Research Communications·Masashige BandoTakao Kataoka
Mar 5, 2002·Nature Biotechnology·Peter Mitchell
Nov 26, 2002·The Journal of Investigative Dermatology·Ayako Nakazono-KusabaToshiyuki Sasaguri
Mar 21, 2003·Biochemical and Biophysical Research Communications·Seunghyi KookWoo Keun Song
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tina WachterRalf W Denfeld

❮ Previous
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Citations

Jul 5, 2007·Science in China. Series C, Life Sciences·ChunChao ZhangLanJuan Li
Jun 1, 2005·Biochemical and Biophysical Research Communications·Ryungsa Kim
May 2, 2009·Cell Death and Differentiation·Y ChenS B Gibson
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Valentina Perissi, Michael G Rosenfeld
Feb 9, 2007·Molecular Biology of the Cell·Michelle E Farah, David C Amberg
Aug 22, 2008·Journal of Cell Science·Changhui WangJeffrey Field
May 26, 2009·Biochemical and Biophysical Research Communications·Qinxi LiBin Jiang
Jun 12, 2010·Annals of the New York Academy of Sciences·Gabriel SosneHoward Matthew
Oct 17, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ying JiangJun Yang
May 13, 2005·Mechanisms of Ageing and Development·Marina JendrachJürgen Bereiter-Hahn
Dec 7, 2013·Journal of Receptor and Signal Transduction Research·Abida ArshadMuhammad Arshad
May 27, 2015·Biochemical and Biophysical Research Communications·Meng XuHuamin Zhou
May 30, 2006·Biochimica Et Biophysica Acta·Vasiliki Anesti, Luca Scorrano
Nov 24, 2005·American Journal of Physiology. Renal Physiology·Xiaoming ZhouMaurice B Burg
Mar 16, 2006·The Biochemical Journal·Ho Lam TangHong Lok Lung
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Campbell W Gourlay, Kathryn R Ayscough
Nov 18, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Roger GodschalkFrederik Jan van Schooten
Jan 5, 2007·Molecular and Cellular Biochemistry·Ramatis Birnfeld de OliveiraJosé Cláudio Fonseca Moreira
Mar 16, 2011·Journal of Leukocyte Biology·Hui ChenZhuoya Li
Aug 14, 2015·International Reviews of Immunology·Porkodi Panneerselvam, Jeak Ling Ding
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna De GeerJelena Levitskaya
Aug 26, 2021·Journal of Cellular Biochemistry·Ferdinand F FatigaChi-Yu Fu

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