Beta-adrenergic receptor mediated protection against doxorubicin-induced apoptosis in cardiomyocytes: the impact of high ambient glucose.

Endocrinology
Naohiro YanoYi-Tang Tseng

Abstract

Recent studies have demonstrated that the beta2-adrenergic receptor (beta2AR)-Galphai signaling pathway exerts a cardiac antiapoptotic effect. The goals of this study were to determine the intracellular signaling factors involved in beta2AR-mediated protection against doxorubicin-induced apoptosis in H9c2 cardiomyocyte and explore the impact of high ambient glucose on the antiapoptotic effect. Under physiological glucose environment (100 mg/dl), beta2AR stimulation prevented doxorubicin-induced apoptosis, which was attenuated by cotreatment with wortmannin, a phosphoinositide 3-kinase (PI3K) inhibitor, or transfection of a dominant-negative Akt. Inhibition of Src kinase with 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d] pyrimidine or cSrc small interfering RNA 32 also attenuated the antiapoptotic effect. Inhibition of platelet-derived growth factor receptor (PDGFR) with AG1296 reversed the beta2AR-induced antiapoptotic effect. Transfection of an active Src cDNA (Y529F) alone was sufficient to render the cells resistant to apoptosis, and the resistance was blocked by wortmannin. Transfection of an active PI3K minigene (iSH2-p110) alone also induced resistance to apoptosis, and the resistance was reversed by an Akt-inhibi...Continue Reading

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Citations

Nov 9, 2011·PloS One·Yohann BernardLaurent Désaubry
Feb 2, 2016·Biochimica Et Biophysica Acta·Alessandra GhigoEmilio Hirsch
Apr 3, 2012·International Journal of Cardiology·Rishi K SomvanshiUjendra Kumar
Apr 14, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Robert T MalletH Fred Downey
Mar 23, 2019·International Journal of Molecular Sciences·Celina M PollardAnastasios Lymperopoulos
Dec 19, 2020·Pharmacological Reviews·Manuela ZaccoloMiguel J Lobo

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