Beta-adrenergic receptor stimulation and adenoviral overexpression of superoxide dismutase prevent the hypoxia-mediated decrease in Na,K-ATPase and alveolar fluid reabsorption

The Journal of Biological Chemistry
Juan LitvanKaren M Ridge

Abstract

Hypoxia has been shown to cause lung edema and impair lung edema clearance. In the present study, we exposed isolated rat lungs to pO(2) = 40 mm Hg for 60 min or rats to 8% O(2) for up to 24 h and then measured changes in alveolar fluid reabsorption (AFR) and Na,K-ATPase function. Low levels of oxygen severely impaired AFR in both ex vivo and in vivo models. The decrease in AFR was associated with a decrease in Na,K-ATPase activity and protein abundance in the basolateral membranes from peripheral lung tissue of hypoxic rats. Beta-adrenergic agonists restored AFR in rats exposed to 8% O(2) (from 0.02 +/- 0.07 ml/h to 0.59 +/- 0.03 ml/h), which was associated with parallel increases in Na,K-ATPase protein abundance in the basolateral membrane. Hypoxia is associated with increased production of reactive oxygen species. Therefore, we examined whether overexpression of SOD2, manganese superoxide dismutase, would prevent the hypoxia-mediated decrease in AFR. Spontaneously breathing rats were infected with a replication-deficient human type 5 adenovirus containing cDNA for SOD2. An otherwise identical virus that contained no cDNA was used as a control (Adnull). Hypoxic Adnull rats had decreased rates of AFR (0.12 +/- 0.1 ml/h) as com...Continue Reading

References

Oct 1, 1993·Journal of Applied Physiology·D H RutschmanJ I Sznajder
Nov 5, 1997·American Journal of Respiratory Cell and Molecular Biology·C PlanèsC Clerici
Jul 20, 1999·Journal of Applied Physiology·F J SaldíasJ I Sznajder
Sep 3, 1999·American Journal of Respiratory and Critical Care Medicine·M L BarnardJ I Sznajder
May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
May 8, 2000·Journal of Applied Physiology·C Clerici, M A Matthay
Jul 21, 2000·American Journal of Respiratory and Critical Care Medicine·F J SaldíasJ I Sznajder
Feb 22, 2001·Journal of Applied Physiology·Z S AzzamJ I Sznajder
May 24, 2001·American Journal of Respiratory and Critical Care Medicine·L B Ware, M A Matthay
Nov 20, 2001·American Journal of Respiratory Cell and Molecular Biology·M L VivonaC Clerici
Apr 16, 2002·Molecular Biology of the Cell·Karen M RidgeJacob I Sznajder
May 25, 2002·The New England Journal of Medicine·Claudio SartoriUrs Scherrer
Oct 17, 2002·Journal of Applied Physiology·Sadis MatalonDouglas C Eaton
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Jun WangYasunobu Okada
Jun 29, 2004·The European Respiratory Journal·C SartoriU Scherrer
Jul 17, 2004·American Journal of Respiratory and Critical Care Medicine·Zaher S AzzamKaren M Ridge
Aug 23, 2005·Proceedings of the American Thoracic Society·Douglas C EatonLucky Jain

❮ Previous
Next ❯

Citations

May 29, 2007·Intensive Care Medicine·István VadászJacob I Sznajder
Nov 1, 2007·Journal of Bioenergetics and Biomembranes·Emilia LecuonaJacob I Sznajder
Sep 26, 2007·The Journal of General Physiology·Irina Yu PetrushankoAnna Yu Bogdanova
Jun 16, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Tsering StobdanM A Qadar Pasha
Jul 7, 2011·Molecular and Cellular Biology·Galina A GusarovaJacob I Sznajder
Feb 22, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Thu P HuynhAyyappan K Rajasekaran
Oct 25, 2008·American Journal of Respiratory and Critical Care Medicine·Alejandro P ComellasJacob I Sznajder
Aug 11, 2007·American Journal of Respiratory Cell and Molecular Biology·Jiwang ChenJacob I Sznajder
Aug 22, 2007·American Journal of Respiratory Cell and Molecular Biology·Gökhan M Mutlu, Phillip Factor
Sep 9, 2008·American Journal of Respiratory Cell and Molecular Biology·Shelley X L ZhangYang Wang
Mar 17, 2009·American Journal of Respiratory Cell and Molecular Biology·Emilia LecuonaJacob I Sznajder
May 13, 2011·American Journal of Respiratory Cell and Molecular Biology·Emel BalogluHeimo Mairbäurl
Jan 26, 2013·American Journal of Respiratory Cell and Molecular Biology·Emilia LecuonaJacob I Sznajder
Oct 8, 2009·Journal of Cell Science·Emilia LecuonaJacob I Sznajder
Nov 29, 2007·PloS One·Arturo BrivaJacob I Sznajder
Jul 6, 2010·Proceedings of the American Thoracic Society·Gary W Hoyle
Jul 6, 2012·Toxicology and Applied Pharmacology·Weiyuan ChangGary W Hoyle
Jul 12, 2011·Critical Care Clinics·Xin Lin, David A Dean
Oct 12, 2010·Free Radical Biology & Medicine·Margaret B ClarkeEva Nozik-Grayck
May 19, 2009·Translational Research : the Journal of Laboratory and Clinical Medicine·Alejandro P Comellas, Arturo Briva
Dec 29, 2007·Biochemical and Biophysical Research Communications·Xu-Jun QinChun-Xu Hai
May 10, 2011·Biochemical and Biophysical Research Communications·Kristina EnelingAlejandro M Bertorello
Dec 23, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Emel BaloğluHeimo Mairbäurl
Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Natalia D MagnaniJacob I Sznajder
Dec 19, 2015·Physiological Reviews·Xin Gen LeiElias S J Arnér
Apr 22, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yochai AdirKaren M Ridge
Dec 13, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guofei ZhouJacob I Sznajder
Nov 10, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ni NaKaren M Ridge

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.