Beta-adrenergic stimulation of skeletal muscle HSL can be overridden by AMPK signaling

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Matthew J WattMark A Febbraio

Abstract

Hormone-sensitive lipase (HSL), an important regulatory enzyme for triacylglycerol hydrolysis within skeletal muscle, is controlled by beta-adrenergic signaling as well as intrinsic factors related to contraction and energy turnover. In the current study, we tested the capacity of 5'AMP-activated protein kinase (AMPK) to suppress beta-adrenergic stimulation of HSL activity. Eight male subjects completed 60 min of cycle exercise at 70% VO2 peak on two occasions: either with normal (CON) or low (LG) pre-exercise muscle glycogen content, which is known to enhance exercise-induced AMPK activity. Muscle samples were obtained before and immediately after exercise. Pre-exercise glycogen averaged 375 +/- 35 and 163 +/- 27 mmol x kg(-1) dm for CON and LG, respectively. AMPK alpha-2 was not different between trials at rest and was increased (3.7-fold, P<0.05) by exercise during LG only. HSL activity did not differ between trials at rest and increased (0 min: 1.67 +/- 0.13; 60 min: 2.60 +/- 0.26 mmol x min(-1) x kg(-1) dm) in CON. The exercise-induced increase in HSL activity was attenuated by AMPK alpha-2 activation in LG. The attenuated HSL activity during LG occurred despite higher plasma epinephrine levels (60 min: CON, 1.96 +/- 0.29 ...Continue Reading

References

Aug 1, 1974·Analytical Biochemistry·J V Passonneau, V R Lauderdale
Feb 15, 2000·The Journal of Biological Chemistry·G M CliffordS J Yeaman
Jul 13, 2000·Biochemical and Biophysical Research Communications·N FujiiL J Goodyear
Aug 15, 2000·Annual Review of Nutrition·C HolmJ A Contreras
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·Z P ChenB E Kemp
Oct 29, 2000·Journal of Applied Physiology·Z GuoM D Jensen
Nov 30, 2000·American Journal of Physiology. Endocrinology and Metabolism·K KawanakaJ O Holloszy
Oct 3, 2001·The Journal of Biological Chemistry·A S GreenbergF B Kraemer
Nov 22, 2001·The Journal of Biological Chemistry·Guenter HaemmerleRudolf Zechner
Jun 11, 2002·Annual Review of Nutrition·David E KelleyLen Storlien
Sep 18, 2002·Journal of Applied Physiology·Matthew J WattLawrence L Spriet
Nov 5, 2002·Biochemical and Biophysical Research Communications·Jørgen F P WojtaszewskiHenriette Pilegaard
Dec 19, 2002·American Journal of Physiology. Endocrinology and Metabolism·Jorgen F P WojtaszewskiErik A Richter
Mar 4, 2003·Journal of Applied Physiology·Matthew J WattLawrence L Spriet
May 22, 2003·American Journal of Physiology. Endocrinology and Metabolism·Matthew J WattDavid J Dyck

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Citations

Nov 7, 2008·Clinical and Experimental Pharmacology & Physiology·Matthew J Watt
Oct 28, 2008·Journal of Lipid Research·Rudolf ZechnerAchim Lass
May 2, 2018·Biological & Pharmaceutical Bulletin·Qi WangGuizhen Tian
Jul 12, 2005·Exercise and Sport Sciences Reviews·Morten DonsmarkHenrik Galbo
Apr 11, 2006·Nature Medicine·Matthew J WattGregory R Steinberg
Sep 29, 2005·American Journal of Physiology. Endocrinology and Metabolism·Matthew J WattMark A Febbraio
Dec 24, 2005·Physiological Reviews·Bente Kiens
May 4, 2006·American Journal of Physiology. Endocrinology and Metabolism·Robert S Lee-YoungGlenn K McConell
Dec 21, 2016·The British Journal of Nutrition·Kurt A EscobarChad M Kerksick
May 13, 2006·The Journal of Physiology·Sebastian B JørgensenJørgen F P Wojtaszewski
May 13, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jason L TalanianLawrence L Spriet
Apr 29, 2006·The Journal of Physiology·Matthew J WattMark A Febbraio
Jul 11, 2006·American Journal of Physiology. Endocrinology and Metabolism·Carsten RoepstorffBente Kiens
Jun 14, 2006·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Gregory R SteinbergBruce E Kemp
Sep 26, 2006·Canadian Journal of Physiology and Pharmacology·Gregory R SteinbergBruce E Kemp
Dec 25, 2008·American Journal of Physiology. Endocrinology and Metabolism·Thomas J AlstedBente Kiens
May 19, 2009·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Gregory R Steinberg
May 19, 2009·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Matthew J Watt
Jun 6, 2009·American Journal of Physiology. Endocrinology and Metabolism·Elisabeth HuijsmanMatthew J Watt
Jul 9, 2009·Physiological Reviews·Gregory R Steinberg, Bruce E Kemp
Aug 21, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ruth C R MeexMatthijs K C Hesselink
Oct 17, 2013·The Journal of Physiology·Matthew J Watt
Dec 6, 2006·Essays in Biochemistry·John A HawleyJuleen R Zierath
Aug 23, 2008·The Biochemical Journal·Matthew J Watt, Gregory R Steinberg
Oct 31, 2018·International Journal of Molecular Sciences·Natalie R JanzenNolan J Hoffman
Apr 9, 2010·Annual Review of Nutrition·Christopher S ShawAnton J M Wagenmakers
Jun 21, 2012·American Journal of Physiology. Endocrinology and Metabolism·Rachael R MasonMatthew J Watt
Apr 3, 2018·Journal of Nutrition and Metabolism·Vincent J MillerJeff S Volek
Nov 16, 2007·American Journal of Physiology. Endocrinology and Metabolism·Cédric MoroSteven R Smith
Jan 3, 2021·Progress in Lipid Research·Emeline RecazensDominique Langin
Dec 30, 2020·Biomolecules·Antonella MuscellaSanto Marsigliante
Jul 14, 2012·Journal of Molecular and Cellular Cardiology·Petra C KienesbergerJason R B Dyck
Feb 26, 2008·Neurologic Clinics·Michaela C Devries, Mark A Tarnopolsky
Dec 17, 2008·Physical Medicine and Rehabilitation Clinics of North America·Michaela C Devries, Mark A Tarnopolsky

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