PMID: 9435585Jan 22, 1998Paper

Beta-adrenoceptor-coupled Gs protein facilitates the activation of cAMP-dependent cardiac Cl- current

The American Journal of Physiology
S PelzerD J Pelzer

Abstract

Here a comparison is made between adenosine 3',5'-cyclic monophosphate (cAMP)-activated Cl- current (ICl) density and activation time course in response to beta-adrenoceptor stimulation with isoproterenol and adenylyl cyclase activation with forskolin. Saturating concentrations of isoproterenol and forskolin failed to activate an ICl in guinea pig atrial as well as in rat and frog ventricular cardiomyocytes. In guinea pig ventricular cardiomyocytes, step application of 1 microM isoproterenol induced an ICl of -0.89 +/- 0.32 pA/pF (holding potential -40 mV, temperature 22 +/- 1 degrees C). ICl activation started after 3 +/- 1 s, was complete within 44 +/- 9 s, and was abolished after cell dialysis with the Rp diastereomer of adenosine 3',5'-cyclic monophosphothioate. Stimulation with increasing concentrations of forskolin (0.01-10 microM) increased ICl density and accelerated ICl activation. With 1 microM forskolin, ICl density was maximal (-0.57 +/- 0.30 pA/pF) but significantly smaller than that achieved with 1 microM isoproterenol. Although ICl density could not be further augmented by forskolin > 1 microM, current activation (latency 28 +/- 8 s, full activation after 112 +/- 8 s with 1 microM forskolin) was further accelerat...Continue Reading

References

Apr 1, 1992·The Journal of General Physiology·T C HwangD C Gadsby
Sep 1, 1991·The American Journal of Physiology·J R Hume, R D Harvey
Jan 1, 1990·Annual Review of Biochemistry·S S TaylorW Yonemoto
Jan 1, 1988·Progress in Biophysics and Molecular Biology·H C Hartzell
May 26, 1989·Science·R D Harvey, J R Hume
Jun 1, 1989·The American Journal of Physiology·K OnoM Arita
Jul 22, 1988·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P Cohen
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Oct 1, 1982·Pflügers Archiv : European journal of physiology·G Isenberg, U Klöckner
Jan 1, 1995·Annual Review of Physiology·D C GadsbyT C Hwang
Apr 1, 1995·Journal of Cardiovascular Electrophysiology·J R Hume, B Horowitz

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Citations

Oct 7, 2005·American Journal of Physiology. Cell Physiology·Xue LinSatoshi Matsuoka

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