Beta-amyloid peptide induces tumor necrosis factor-alpha and nitric oxide production in murine macrophage cultures

Neuroreport
F ShalitM Huberman

Abstract

We investigated the effect of beta-amyloid peptide (betaA) on the activation of the murine-derived monocyte/macrophage J774 cell-line. BetaA induced tumor necrotic factor-alpha (TNF alpha) in these cells in a dose-dependent manner. Incubation of cells with betaA slightly increased nitric oxide (NO) production, an effect that was significantly enhanced by the addition of interferon-gamma (IFN gamma). Substitution of betaA4 with TFN alpha and incubation of the cultures with IFN gamma resulted in significant NO production, although this was lower than that obtained in the presence of the peptide. Incubation of cultures with a monoclonal antibody (mAb) against TNF alpha abrogated NO production. Our results suggest that betaA4-induced TNF alpha production is a crucial event in the activation of peripheral macrophages.

Citations

Jan 5, 2002·Neurobiology of Aging·R T PerryR C Go
Jul 19, 2005·Immunity & Ageing : I & a·Simona BussiniPierluigi Baron
Jul 5, 2005·Neurobiology of Aging·Giovanna SebastianiPatrick Tremblay
May 11, 2005·Biochemical and Biophysical Research Communications·Jung-Hee Jang, Young-Joon Surh
Dec 19, 2012·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Hk ElçioğluMa Karan

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