Beta-amyloid stimulates murine postnatal and adult microglia cultures in a unique manner.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Angela M Floden, Colin K Combs

Abstract

Reactive microglia are commonly observed in association with the beta-amyloid (Abeta) plaques of Alzheimer's disease brains. This localization supports the hypothesis that Abeta is a specific activating stimulus for microglia. A variety of in vitro studies have used postnatal derived rodent microglia cultures to characterize the ability of Abeta to stimulate these cells. However, it is unclear whether this paradigm accurately models conditions in aged animals. To determine whether Abeta stimulatory phenotypes differ between young and adult microglia, we established cultures of acutely isolated adult murine cortical microglia to compare with postnatal derived microglial cultures. Although cells from both ages expressed robust immunoreactivity for CD68 and CD11b, their responses to activating stimuli differed. Fibrillar Abeta was rapidly phagocytosed by postnatal microglia and both oligomeric and fibrillar peptide stimulated increased tumor necrosis factor alpha (TNFalpha) secretion. However, Abeta oligomers but not fibrils stimulated TNFalpha secretion from adult microglia. More importantly, adult microglia had diminished ability to phagocytose Abeta fibrils. These findings demonstrate that adult microglia respond to Abeta fibri...Continue Reading

Citations

Aug 19, 2011·Experimental Brain Research·D R HowlettC J Hille
Jul 16, 2010·Journal of Neural Transmission·Michael T HenekaMarkus Peter Kummer
Nov 30, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kendra L PuigColin K Combs
Dec 15, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Raasay S JonesMarina A Lynch
Jul 12, 2008·Nature Reviews. Drug Discovery·Mike Dragunow
Jul 5, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Aaron C HirkoJeffery A Hughes
Dec 18, 2013·Journal of Neuroinflammation·Arnaud FrançoisGuylène Page
Nov 5, 2011·Journal of Neuroinflammation·Tiina M KauppinenRaymond A Swanson
Aug 6, 2013·Biological & Pharmaceutical Bulletin·Daisuke OzawaMasatoshi Beppu
Sep 25, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erin G Reed-GeaghanGary E Landreth
Feb 27, 2013·Current Translational Geriatrics and Experimental Gerontology Reports·Tarja MalmJari Koistinaho
Mar 15, 2016·Neurobiology of Aging·Kendra L PuigColin K Combs
Mar 29, 2013·Neuroscience·M Szabo, K Gulya
Jul 24, 2010·Neuropharmacology·Grant A Krafft, William L Klein
Jan 12, 2010·Brain, Behavior, and Immunity·K McQuillanKingston H G Mills
Oct 17, 2009·Neurobiology of Disease·Brent Cameron, Gary E Landreth
May 2, 2008·Experimental Neurology·Laura Gasparini, Alexander Dityatev
Apr 16, 2008·Journal of Neurochemistry·Tammy Kielian
Jul 9, 2013·Glia·Clara BeutnerHarald Neumann
Jul 23, 2011·Journal of Cellular and Molecular Medicine·Johanna MaggaJari Koistinaho
Dec 17, 2009·Journal of Neurochemistry·Rommy von BernhardiJaime Eugenín
Feb 19, 2016·Journal of Alzheimer's Disease : JAD·Katherine A SouthamDavid H Small
Nov 23, 2013·Neurobiology of Aging·Juliet M TaylorPeter J Crack
Jun 6, 2013·Mediators of Inflammation·Francisca Cornejo, Rommy von Bernhardi
Mar 19, 2011·Neurobiology of Aging·Maria Teresa FerrettiA Claudio Cuello
Mar 11, 2015·Biochemistry·Michael R NicholsShana E Terrill-Usery
Jun 5, 2015·Frontiers in Aging Neuroscience·Fernanda G De Felice, Mychael V Lourenco

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