beta-Carboline alkaloids as matrices for UV-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in positive and negative ion modes. Analysis of proteins of high molecular mass, and of cyclic and acyclic oligosaccharides

Rapid Communications in Mass Spectrometry : RCM
H NonamiR Erra-Balsells

Abstract

We report that commercially available beta-carbolines (nor-harmane (9H-pyrido[3,4-b]indole), harmane (1-methyl-9H-pyrido[3,4-b]indole), harmine (7-methoxy-1-methyl-9H-pyrido[3,4-b]indole), harmol (1-methyl-9H-pyrido[3,4-b]indol-7-ol), harmaline (3,4-dihydro-7-methoxy-1-methyl-9H-pyrido[3,4-b]indole) and harmalol (3,4-dihydro-1-methyl-9H-pyrido[3,4-b]indol-7-ol)), are useful MALDI matrices at 337 nm, for cyclic oligosaccharides (cyclodextrins, range 972-1290 Da), acyclic oligosaccharides (range 342-828 Da) and high molecular mass proteins (range 23,290-66,525 Da) in both positive and negative modes. This was investigated by using time-of-flight (TOF) mass spectrometers of different sensitivities, equipped with and without pulse extraction facilities. A comparison with conventional matrices for carbohydrates (DHB and DHB/HIC) indicates that beta-carbolines provide the same level of sensitivity and resolution in the positive mode, but offer the advantage of high levels of sensitivity and resolution in the negative mode. Harmaline has been found to be specially effective for the analysis of high-mass proteins in both modes, and also exhibits excellent experimental reproducibility of the results owing to the homogeneous crystallizat...Continue Reading

Citations

Dec 9, 2008·Journal of the American Chemical Society·Sung Hwan YoonVicki H Wysocki
Sep 10, 2014·Journal of Agricultural and Food Chemistry·Yoon Hee KimHirofumi Tachibana
Dec 2, 2014·Journal of Agricultural and Food Chemistry·María F BasantaCarlos A Stortz
May 10, 2011·Journal of Pharmacological and Toxicological Methods·Hannah BatesonChris W Sutton
Nov 13, 2004·Rapid Communications in Mass Spectrometry : RCM·David J HarveyChris W Sutton
Apr 30, 2003·Rapid Communications in Mass Spectrometry : RCM·Shaoxiang XiongGuanghui Wang
Jun 17, 2006·Rapid Communications in Mass Spectrometry : RCM·Adriana C CasabuonoAlicia S Couto
Jun 5, 2007·Rapid Communications in Mass Spectrometry : RCM·Nien-Yeen HsuChung-Hsuan Chen
Aug 2, 2008·Journal of Mass Spectrometry : JMS·Daiki AsakawaKenzo Hiraoka
Feb 27, 2008·Journal of Mass Spectrometry : JMS·Ichiro KudakaKenzo Hiraoka
Mar 26, 2011·Journal of Mass Spectrometry : JMS·Yu-Ling ChangChung-Hsuan Chen
Mar 24, 2016·European Journal of Pharmacology·Masaomi TerajimaYasuyuki Higashi
May 15, 2007·Molecular and Biochemical Parasitology·Malena LandoniAlicia S Couto
Feb 14, 2002·Chemical Reviews·Yehia Mechref, Milos V Novotny
Mar 24, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Gabriela PetroselliGabriel M Bilmes
Jul 30, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M Micaela GonzalezRosa Erra-Balsells
Sep 21, 2012·Journal of the American Society for Mass Spectrometry·David J HarveyJames H Scrivens
Apr 25, 2020·Mass Spectrometry Reviews·David J Harvey
Nov 1, 2012·Organic & Biomolecular Chemistry·M Micaela GonzalezFranco M Cabrerizo
May 7, 2021·Mass Spectrometry Reviews·Alba HykollariIain B H Wilson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.