Beta-catenin signalling in mesenchymal islet-derived precursor cells.

Cell Proliferation
Laertis IkonomouMarvin C Gershengorn

Abstract

Previously, we characterized human islet-derived precursor cells (hIPCs) as mesenchymal stem cells that migrate out from islets in vitro and can differentiate into functional islet-like structures following proliferative expansion. Here, we investigate the role of beta-catenin signalling in derivation and proliferation of hIPCs. Localization of beta-catenin was performed using confocal microscopy. Expression levels of beta-catenin target genes were measured by quantitative real-time polymerase chain reaction. Loss-of-function studies were performed using specific short interfering RNAs. Immunostaining of islet outgrowths revealed translocation of beta-catenin from plasma membranes in intact islets to the nucleus in cells migrating out. There were no nuclear beta-catenin-positive cells in intact islets whereas between 35% and 70% of cells in established hIPC cultures exhibited nuclear beta-catenin. Transcripts for beta-catenin target genes were increased in hIPCs compared to those in islets. Beta-catenin translocated to the cell membrane when hIPCs formed epithelial cell clusters. In proliferating hIPCs, there was a strong correlation between markers of proliferation and nuclear beta-catenin. Treatment of hIPCs with the glycogen...Continue Reading

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Citations

Sep 17, 2008·The Review of Diabetic Studies : RDS·Shimon Efrat
Oct 17, 2013·Journal of Cellular Physiology·Sahar Hiram-BabYoram Oron
Aug 29, 2020·Diabetes/metabolism Research and Reviews·Bushra MemonEssam M Abdelalim
Jan 30, 2009·Journal of Cellular and Molecular Medicine·Behrous DavaniMarvin C Gershengorn

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