PMID: 2107872Mar 26, 1990Paper

Beta-D-glycosidase activities of Humicola grisea: biochemical and kinetic characterization of a multifunctional enzyme

Biochimica Et Biophysica Acta
R M PeraltaJ A Jorge

Abstract

A beta-D-glycosidase activity was purified from mycelium of Humicola grisea var. thermoidea grown on avicel as the main carbon source. The purified enzyme was a glycoprotein and migrated as a single polypeptide band on polyacrylamide gel electrophoresis under native or denaturing conditions. The apparent molecular weight of the enzyme was estimated to be 55 kDa by gel filtration and SDS-PAGE. The enzyme was active against o-nitrophenyl beta-D-galactoside; p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-fucoside, lactose and cellobiose, PNP fucoside (synthetic substrate) and cellobiose (natural substrate) being the best utilized. A comparison of the properties of beta-D-galactosidase, beta-D-glucosidase and beta-D-fucosidase showed that three activities exhibited similar pH and temperature optima and the same thermostability. The hydrolysis rate of substrate mixtures suggests that the enzyme possesses a common catalytic site for all the substrates assayed.

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Citations

May 1, 1996·World Journal of Microbiology & Biotechnology·M L PolizeliH F Terenzi
Apr 6, 2005·Biochimica Et Biophysica Acta·Agda Karina B Lúcio-EterovicHéctor F Terenzi
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·R MaheshwariM K Bhat
Dec 7, 2013·World Journal of Microbiology & Biotechnology·Marielle Aleixo GiraldoLuis Henrique Souza Guimarães
Mar 15, 2014·Applied Biochemistry and Biotechnology·Luana Parras MeleiroRosa Prazeres Melo Furriel
Aug 12, 2009·Applied Biochemistry and Biotechnology·Bruno BenolielLidia Maria Pepe de Moraes
Oct 17, 2008·Journal of Industrial Microbiology & Biotechnology·Simone de Carvalho Peixoto-NogueiraMaria de Lourdes Teixeira de Moraes Polizeli
Nov 4, 2004·FEMS Microbiology Letters·Fabiana Fonseca ZanoeloJoão Atílio Jorge
Sep 13, 2013·Journal of Basic Microbiology·Alexandre MallerMaria de Lourdes Teixeira de Moraes Polizeli
Dec 6, 1996·Biochimica Et Biophysica Acta·M K KadowakiJ A Jorge
Nov 21, 1997·Biochimica Et Biophysica Acta·S LeparouxB Colas
Jun 1, 1995·Applied and Environmental Microbiology·L Campos, C R Felix
May 1, 1991·Applied and Environmental Microbiology·C RiouM Fevre
Dec 2, 2021·Journal of Applied Microbiology·Letícia Mara RasboldAlexandre Maller

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